The larval mosquito midgut exhibits one of the highest pH values known in a biological system. While the pH inside the posterior midgut and gastric caeca ranges between 7.0 and 8.0, the pH inside the anterior midgut is close to 11.0. Alkalization is likely to involve bicarbonate/carbonate ions. These ions are produced in vivo by the enzymatic action of carbonic anhydrase. The purpose of this st...

Citation: Koh CZY, Hiong KC, Choo CYL, Boo MV, Wong WP, Chew SF, Neo ML and Ip YK (2018) Molecular Characterization of a Dual Domain Carbonic Anhydrase From the Ctenidium of the Giant Clam, Tridacna squamosa, and Its Expression Levels After Light Exposure, Cellular Localization, and Possible Role in the Uptake of Exogenous Inorganic Carbon. Front. Physiol. 9:281. doi: 10.3389/fphys.2018.00281 M...

Existence of an internal carbonic anhydrase was demonstrated in the cyanobacterium Synechocystis PCC 6714. The enzyme, present at a low specific activity, was inducible by limitation in inorganic carbon and inhibited both in vivo and in vitro by acetazolamide. The internal inorganic carbon pool as determined by mass spectrometry, was similarly modulated by the actual inorganic carbon growth reg...

Capsaicin, the component responsible for the pungency of chili peppers, shows beneficial effects in many diseases, although the underlying mechanisms remain unclear. In the present study, the potential targets of capsaicin were predicted using PharmMapper and confirmed via chemical-protein interactome (CPI) and molecular docking. Carbonic anhydrase 2 was identified as the main disease-related t...

Mgz+. The enzyme assays were accompanied by measurements of the phosphorylation with [pJZP]ATP. After incubation of intact rabbit erythrocytes with adrenaline (Fig. l), maximal activation of carbonic anhydrase occurred within 5min. In chicken erythrocytes, one of the two isolated isoenzymes could be activated and phosphorylated (for conditions, see the legend of Fig. 2). The carbonic anhydrase ...