The purpose of this study is to evaluate in vitro gene delivery mediated by asialofetuin-appended cationic liposomes (AF-liposomes) associating cyclodextrins (CyD/AF-liposomes) as a hepatocyte-selective nonviral vector. Of various CyDs, AF-liposomes associated with plasmid DNA (pDNA) and γ-cyclodextrin (γ-CyD) (pDNA/γ-CyD/AF-liposomes) showed the highest gene transfer activity in HepG2 cells wi...

The proteoglycan decorin modulates leukocyte recruitment during delayed-type hypersensitivity (DTH) responses. Decorindeficient (Dcn) mice show reduced edema formation during the first 24 h with a concurrent attenuated recruitment of Cd8 leukocytes in the inflamed Dcn ears. The aim of this study was to elucidate the molecular pathways affected by the loss of decorin. In vivo, reduced numbers of...

The effect of oleic, linoleic and γ-linolenic acids on ROS production by 3T3 Swiss and Rat 1 fibroblasts was investigated. Using lucigenin-amplified chemiluminescence, a dose-dependent increase in extracellular superoxide levels was observed during the treatment of fibroblasts with oleic, linoleic and γ-linolenic acids. ROS production was dependent on the addition of β-NADH or NADPH to the medi...

Milk was co-fermented with Bacillus subtilis HA and Lactiplantibacillus plantarum EJ2014 to produce a dairy ingredient enriched poly-γ-glutamic acid (γ-PGA) γ-aminobutyric (GABA). The first fermentation of milk B. resulted in viscous broth pH 6.56, 0.26% acidity, 1.40 mg/g tyrosine equivalent, 17.21 U/g protease activity. viable cell counts indicated 8.74 log CFU/mL, the consistency index alkal...

interferon gamma (ifn-γ) is one of the key cytokines in defining t helper 1 lymphocyte immuneresponses. in this study, the bovine ifn-γ gene was cloned from spleen tissue rna using the reversetranscription-polymerase chain reaction (rt-pcr). ifn-γ cdna was sub-cloned and expressed inmammalian expression plasmid (pcdna3.1(+)) under the control of the human cytomegalovirus (cmv)promoter. the pred...

to investigate the antifungal effects of essential oils of khuzestani savory (satureja khuzistanica jamzad), rechingeri savory (s. rechingeri jamzad), carvacrol and benomyl on botrytis cinerea, this study was carried out in a pda medium, and on grapes, kiwi fruits and strawberry. the experiment was laid out factorially based on a completely randomized design with three replications. the results...

We determine the asymptotic behaviour of (bilateral) obstacle problems for fractional energies in rather general aperiodic settings via Γ-convergence arguments. As further developments we consider obstacles with random sizes and shapes located on points of standard lattices, and the case of random homothetics obstacles centered on random Delone sets of points. Obstacle problems for non-local en...

In this paper, a class of minimization problems, labeled by an index 0 < h < 1, is considered. Each minimization problem is for a freeenergy, motivated by the magnetics in 3D-ferromagnetic thin film, and in the context, the index h denotes the thickness of the observing film. The Main Theorem consists of two themes, which are concerned with the study of the solvability (existence of minimizers)...

The behavior of a thin curved hyperelastic film bonded to a fixed substrate is described by an energy composed of a nonlinearly hyperelastic energy term and a debonding interfacial energy term. We compute the Γ-limit of this energy under a noninterpenetration constraint that prohibits penetration of the film into the substrate without excluding contact between them.

We are interested in the homogenization of elastic-electric coupling equation, with rapidly oscillating coefficients, in periodically perforated piezoelectric body. We justify the two first terms in the usual asymptotic development of the problem solution. For the main convergence results of this paper, we use the notion of two-scale convergence. A two-scale homogenized system is obtained as th...