Recent development of DNA markers provides powerful tools for population genetic analyses. Amplified fragment length polymorphism (AFLP) markers result from a polymerase chain reaction (PCR)-based DNA fingerprinting technique that can detect multiple restriction fragments in a single polyacrylamide gel, and thus are potentially useful for population genetic studies. Because AFLP markers have to...

A glycoprotein (Cpgp40/15)-encoding gene of Cryptosporidium parvum was analyzed to reveal intraspecies polymorphism within C. parvum isolates. Forty-one isolates were collected from different geographical origins (Japan, Italy, and Nepal) and hosts (humans, calves, and a goat). These isolates were characterized by means of DNA sequencing, PCR-restriction fragment length polymorphism (PCR-RFLP),...

The Italian-native Mediterranean brown trout (Salmo ghigii) is a seriously threatened freshwater fish, especially by anthropogenic hybridisation with the domestic strains of Atlantic origin that have been repeatedly released into wild for angling. A PCR-restriction fragment length polymorphism (RFLP) assay diagnostic lactate dehydrogenase chain-1 (LDH-C1) gene sequences has routinely applied to...

Toxoplasma gondii pathogenicity depends on the type derived from a clonal population. A genetic analysis of locus has been carried out to determine different genotypes T. (strain types I, II, and III) that are associated with human toxoplasmosis. The several linked toxoplasmosis have identified through study locus. In this investigation, PCR-RFLP was found be useful, simple method genotypic cha...

Recently, restriction fragment length polymorphism (RFLP) of alpha 2-adrenergic receptor gene (alpha 2-C10) digested with Bsu36I restriction enzyme has been reported in US populations. Therefore, we examined the association of this RFLP with essential hypertension by comparing the frequency of specific alleles for this gene in Japanese populations. The distribution of this RFLP was compared wit...

A quantitative fingerprinting method, called the real-time terminal restriction fragment length polymorphism (real-time-t-RFLP) assay, was developed for simultaneous determination of microbial diversity and abundance within a complex community. The real-time-t-RFLP assay was developed by incorporating the quantitative feature of real-time PCR and the fingerprinting feature of t-RFLP analysis. T...

A pyrosequencing protocol was developed as a rapid and reliable method to identify the mutations of the dhfr and dhps genes of Plasmodium falciparum that are associated with antifolate resistance. The accuracy and specificity of this method were tested using six laboratory-cultured P. falciparum isolates harboring known single nucleotide polymorphisms (SNPs) in the genes dhfr (codons 50, 51, 59...

A quantitative molecular technique was developed for rapid analysis of microbial community diversity in various environments. The technique employed PCR in which one of the two primers used was fluorescently labeled at the 5' end and was used to amplify a selected region of bacterial genes encoding 16S rRNA from total community DNA. The PCR product was digested with restriction enzymes, and the...

BACKGROUND Dermatophytes are the most common causative agents of superficial mycoses. Species identification of these fungi is important from therapeutic and epidemiological point of wive. Traditional approaches for identification of dermatophytes at the species level, relying on macroscopic and microscopic features of the colonies, usually are time-consuming and unreliable in many circumstance...

هدف: تخمین شناختی تلاش برای ارائه یک پاسخ معقول براساس اطلاعات در دسترس است. هدف از این مطالعه طراحی آزمون و بررسی روایی، پایایی مقادیر هنجار آن روش: 643 نفر دانشجویان دانشگاه شهید بهشتی انجام شد. آزمون‌های شده حاضر، مرتب کردن کارت­‌های ویسکانسین، رنگ واژه استروپ حافظه فعال چند محرک پیشین به عنوان آزمون­‌های کارکرد اجرایی همزمان ارزیابی مورد استفاده قرار گرفت. همبستگی پیرسون روایی همزمان، تی مق...