One of the major mechanisms involved in acetaminophen (APAP)induced hepatotoxicity is hepatocyte nuclear factor 4a (HNF4a)mediated activation of pregnane X receptor (PXR) and constitutive androstane receptor (CAR). In the present study, we investigated the role of miR-561 and its target gene DAX-1 encoding a corepressor of HNF4a in the process of APAP-induced hepatotoxicity. We used both human ...