In this article, we describe the kinetic ELISA of Blue Tongue and Epizootic Hemorrhagic Disease viral antibodies in microfluidic channels by monitoring the rate of generation of the enzyme reaction product under static conditions. It has been shown that this format of the immunoassay allows very reliable quantitation of the target species using inexpensive glass microchips and a standard epiflu...

Uveitis and primary intraocular lymphoma (PIOL) are diseases associated with the invasion of lymphocytes into various regions of the eye, accompanied by expression of inflammatory cytokines. While these diseases are very different in terms of survivability and treatment options they have similar symptoms that make accurate diagnosis challenging. Furthermore, the diagnostic yield with state-of-t...

for the evaluation of autoantibodies in pemphigus vulgaris (pv), an indirect elisa assay was developed by using a semi-purified human epidermal skin extracted with approximately a molecular weight (m.w.) of 130-160 kda. the evaluation of pemphigus igg, igm and iga autoantibodies in 75 patients and 50 control sera indicated that this indirect elisa assay was a useful method for the detection of ...

background: patients with prolonged neutropenia and/or severe underlying immunosuppression are at thegreatest risk for disseminated aspergillosis. this study was undertaken to determine the incidence of invasiveaspergillosis by platelia aspergillus enzyme-linked immunosorbent assay elisa kit in high risk children admitted tothe hematology ward of dr. faghihi hospital, shiraz university of medic...

background: toxoplasmosis is a parasitic disease caused by protozoan, toxoplasma gondii. infections of human are common and are usually asymptomatic. the infection may be serious if is transmitted to the fetus during pregnancy. prophylactic measures, early detection of the infection and treatment can avoid congenital toxoplasmosis and many long term effects. objective: seroepidemiological study...

this study was conducted to evaluate and compare the levels of aflatoxin m1 in raw and pasteurized milk samples during different seasons by enzyme- linked immuno sorbent assay in sanandaj, iran. in 257 (94.49%) out of 272 milk samples the presence of aflatoxin m1 was detected in concentrations ranging between 0.007 and 115.930 ng/l. afm1 level in 12 (4.4%) of positive samples were higher than t...

background: enzyme linked immunosorbent assay (elisa) has been described as an alternative to radioimmunoassay for the mammalian and nonmammalian steroids detection. in this study, a simple and rapid elisa is described and validated for 4-pregnen-3,20, dione (progesterone). materials and methods: a general procedure for preparation of the acetylcholinesterase labelled steroid is described which...