Polyhydroxyalkanoates (PHAs) composed of a mixture of short-chain-length-medium-chain-length (SCL-MCL) hydroxyacyl monomers are biologically produced polyesters that have properties ranging from thermoplastic to elastomeric, dependent on the molar ratio of SCL to MCL monomers incorporated into the copolymer. Because of the potential wide range of properties and applications for SCL-MCL PHA copo...

A passive hemagglutination (PHA) assay for antibody to Pseudomonas aeruginosa exotoxin is described which utilizes chromic chloride-treated ovine erythrocytes coated with purified toxin. PHA antitoxin titers correlated well with those obtained by a cytotoxicity neutralization assay (r = 0.91, P less than 0.001), whereas the PHA assay was four to eight times as sensitive. The mean serum PHA tite...

The acetoacetyl-CoA reductase and the polyhydroxyalkanoate (PHA) synthase from Ralstonia eutropha (formerly Alcaligenes eutrophus) were expressed in Escherichia coli, Klebsiella aerogenes, and PHA-negative mutants of R. eutropha and Pseudomonas putida. While expression in E. coli strains resulted in the accumulation of poly(3-hydroxybutyrate) [PHB], strains of R. eutropha, P. putida and K. aero...

The pha-4 locus encodes a forkhead box A (FoxA/HNF3) transcription factor homolog that specifies organ identity for Caenorhabditis elegans pharyngeal cells. We used microarrays to identify pharyngeal genes and analyzed those genes to determine which were direct PHA-4 targets. Our data suggest that PHA-4 directly activates most or all pharyngeal genes. Furthermore, the relative affinity of PHA-4...

BACKGROUND: The primitive hypoglossal artery (PHA) is a rare vascular anomaly, which belongs to the group of carotid-basilar anastomosis that may occur in adults. CASE PRESENTATION: Herein is presented a case of a patient with a PHA, who had undergone a cerebral angiography due to investigation of subarachnoid hemorrhage. Additionally, the diagnostic alternatives for detection and assessment of...

Aerobic anoxygenic phototrophic bacteria (AAPB) are unique players in carbon cycling in the ocean. Cellular carbon storage is an important mechanism regulating the nutrition status of AAPB but is not yet well understood. In this paper, six AAPB species (Dinoroseobacter sp. JL1447, Roseobacter denitrificans OCh 114, Roseobacter litoralis OCh 149, Dinoroseobacter shibae DFL 12(T), Labrenzia alexa...

It is well known that peripheral lymphocytes rarely divide in tissue culture; indeed, they have usually been considered to be the end cell in the pathway of differentiation. However, in the presence of certain mitogenic agents, of which phytohemagglutinin (PHA) is the best known example, a striking transformation occurs; the cells increase their over-all metabolic activity, enlarge, and divide....

BACKGROUND Polyhydroxyalkanoates (PHA) are synthesized by many bacteria in the cytoplasm as storage compounds for energy and carbon. The key enzymes for PHA biosynthesis are PHA polymerases, which catalyze the covalent linkage of 3-hydroxyacyl coenzymeA thioesters by transesterification with concomitant release of CoA. Pseudomonas putida GPo1 and many other Pseudomonas species contain two diffe...

Nhựa sinh học Polyhydroxyalkanoate (PHA) là loại nhựa do vi vật tổng hợp nên trong điều kiện dư thừa nguồn cacbon. Có nhiều có thể PHA, đó các loài chịu mặn như Bacillus, Halomonas... Tuy nhiên, thuộc vùng đảo Trường Sa chưa được quan tâm nghiên cứu. Nghiên cứu này trình bày một số kết quả phân lập và đánh giá khả năng PHA của chủng từ quần Sa. Trong 21 khuẩn TSLT21 tới 30,9% khối tế bào khô đế...

ownloade pose: This dose-escalation study evaluated the safety, tolerability, and pharmacokinetics (PK) of al Src inhibitor saracatinib (AZD0530) in patients with advanced solid malignancies. Tumor biopsy es were taken to investigate the effect of saracatinib on Src activity in tumors. erimental Design: Part A of the study followed a multiple-ascending dose design to establish the um tolerated ...