Umbilical vein catheter (UVC) is used in managing critically sick neonates all over the world. It is generally considered to be safe although various complications can arise and are well known. Herein we describe a successful retrieval of a broken and migrated UVC across the heart in a neonate. Pertinent literature regarding rarity of its occurrence and mechanism of occurrence has been touched ...

Sunlight causes lesions in DNA that if unrepaired and inaccurately replicated by DNA polymerases yield mutations that result in skin cancer in humans. Two enzymes involved in translesion synthesis (TLS) of UV-induced photolesions are DNA polymerase eta (Poleta) and polymerase zeta (Polzeta), encoded by the RAD30A and REV3 genes, respectively. Previous studies have investigated the TLS roles of ...

We previously reported that Shewanella oneidensis MR-1 is highly sensitive to UVC (254 nm), UVB (290 to 320 nm), and UVA (320 to 400 nm). Here we delineated the cellular response of MR-1 to UV radiation damage by analyzing the transcriptional profile during a 1-h recovering period after UVC, UVB, and UVA exposure at a dose that yields about a 20% survival rate. Although the SOS response was obs...

DNA replication is inhibited by DNA damage through cis effects on replication fork progression and trans effects associated with checkpoints. In this study, we employed a combined pulse labeling and neutral-neutral two-dimensional gel-based approach to compare the effects of a DNA damaging agent frequently employed to invoke checkpoints, UVC radiation, on the replication of cellular and simian ...

DNA polymerase eta (poleta) performs translesion synthesis past ultraviolet (UV) photoproducts and is deficient in cancer-prone xeroderma pigmentosum variant (XP-V) syndrome. The slight sensitivity of XP-V cells to UV is dramatically enhanced by low concentrations of caffeine. So far, the biological explanation for this feature remains elusive. Using DNA combing, we showed that translesion synt...

In recent decades, marine reserves have been established either to protect ecosystem structure and biological diversity or to serve as management tools to counter the overexploitation of fish stocks. The Cape Roux marine protected area (MPA), in the Mediterranean Sea, was established in December 2003 for the management of artisanal fisheries and enhancement target fish stocks. Monitoring of lit...

Central venous access is an important aspect of neonatal intensive care management. Malpositioned central catheters have been reported to induce cardiac tachyarrhythmia in adult populations and there are case reports within the neonatal population. We present a case of a preterm neonate with a preexisting umbilical venous catheter (UVC), who then developed a supraventricular tachycardia (SVT). ...

We modelled a 'clean' surgical wound lightly contaminated with airborne bacteria, using agar, ovine muscle and ovine adipose tissue. This was used to assess the effect on bacteria of ultraviolet C light (UVC) 1200 mu W/cm2, hydrogen peroxide 3%, povidone-iodine 1% and 10%, chlorhexidine 0.05%, pulsed jet lavage with UVC and syringe and needle lavage with chlorhexidine 0.05%. All the agents were...

Photocatalytic degradations of azo dye (RR 180), pesticide (2,4-D) and antibiotic (enrofloxacin) in aqueous solutions were performed and compared by using pure ZnO and ZnO/TiO2 composite (at 1:1 ZnO to TiO2 mole ratio) catalysts in a self-supporting plate form. The plates were produced by tape casting of the constituent powder slurries and sintering at 600°C. Photocatalytic degradations of thes...