The nucleic acid-binding proteins of bovine leukemia virus (BLV) and feline leukemia virus (FeLV) were isolated in a high state of purity with chloroform-methanol extraction followed by reversed-phase liquid chromatography. Selective solubilization and purity of BLV p12 and FeLV p10 was confirmed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. The compositions and molecular weight...

Bovine leukemia virus (BLV) is a retrovirus that causes enzootic bovine leucosis. To investigate the presence and genetic variability of BLV in the Caribbean for the first time, we preformed fluorescence resonance energy transfer (FRET)-PCR for the pol of BLV on DNA from whole blood of cattle from Dominica, Montserrat, Nevis and St. Kitts. Standard PCRs with primers for the env were used for ph...

Bovine leukemia virus (BLV) expression is mostly silent in peripheral blood mononuclear cells (PBMCs) of infected animals. However, when infected cells are cultured, they are stimulated to produce virus. We studied viral transcription in PBMCs taken from BLV-infected sheep because the pattern of transcriptional activation in these cells should closely mimic activation of virus expression within...

by the immunodiffusion test was found to be the same as that detected by the IFA technique (5). Previous studies also showed that disruption of the virus is necessary for release of the antigen, suggesting that the antigen is an internal virion component (3, 6). The resistance of the BLV antigen to ether and acetone, and its presence in the cytoplasm of infected cells, are features which also c...

SORIN POROJAN1, LILIANA POROJAN2* 1”V. Babes” University of Medicine and Pharmacy Timisoara, Faculty of Dentistry, Department of Oral Rehabilitation, Specialization Dental Technology, 9 Revolutiei 1989 Blv, Timisoara, Romania 2 ”V. Babes” University of Medicine and Pharmacy Timisoara, Faculty of Dentistry, Department of Prostheses Technology, Specialization Dental Technology, 9 Revolutiei 1989 ...

Background and Aims: Bovine leukaemia virus (BLV) is an oncogenic member of the genus Deltaretrovirus of the family Retroviridae. BLV is the causative agent of enzootic bovine leukaemia and infects cattle worldwide, imposing economic impact on the dairy cattle industry. The purpose of this study was to estimate the seroprevalence of BLV in cattle in some provinces of Iran. Materials and Methods...

BACKGROUND: Bovine leukemia virus (BLV) is an oncogenic deltaretrovirus causing a persistent lifelong infection of B lymphocytes. In addition to the domestic cattle, the virus can also infect water buffaloes. OBJECTIVES: Recent investigations have demonstrated the increasing prevalence of BLV infection among cattle population in Iran. Large popul...

BACKGROUND Human T-cell leukemia virus (HTLV)/bovine leukemia virus (BLV) group retroviruses, which cause hematopoietic cancers, encode a unique protein, Tax, involved in the transformation of infected cells. Our purpose was to determine whether the mechanism by which Tax protein induces transformation in HTLV- or BLV-infected cells involves DNA damage. METHODS We used a micronucleus assay to...

Programmed death-1 (PD-1), an immunoinhibitory receptor on T cells, is known to be involved in immune evasion through its binding to PD-ligand 1 (PD-L1) in many chronic diseases. We previously found that PD-L1 expression was upregulated in cattle infected with bovine leukemia virus (BLV) and that an antibody that blocked the PD-1/PD-L1 interaction reactivated T-cell function in vitro. Therefore...