Fragile X Syndrome is the most common inherited intellectual disability, and Fragile X Syndrome patients often exhibit motor and learning deficits. It was previously shown that the fmr1 knock-out mice, a common mouse model of Fragile X Syndrome, recapitulates this motor learning deficit and that the deficit is associated with altered plasticity of dendritic spines. Here, we investigated the mot...

This work is focused on the development and validation of a spark advance controller, based piston “damage” model predictive knock model. The algorithm represents an integrated innovative way to manage both intensity combustion phase. It characterized by model-based open-loop with capability calculating high accuracy timing that achieves desired damage in certain period, for knock-limited engin...

The factors influencing the onset of knocking have a significant impact on how well SI engine performs. Hence, efficacy in determining and controlling knock is key factor improving engine's performance. This paper provides insight into role block vibrations occurrence using Empirical Mode Decomposition Short-Time Fourier Transform. To comprehend behaviour vibration amid normal combustion condit...

PURPOSE The purpose of this study was to investigate the effect of Sirtuin 1 (SIRT1) and General control nonderepressible 5 (GCN5) knock down on peroxisome proliferator- activated receptor gamma coactivator 1-alpha (PGC-1α) deacetylation during electrical stimulated skeletal muscle contraction. METHODS Skeletal muscle primary cell were isolated from C57BL/6 mice gastrocnemius and transfected ...

BACKGROUND The CRISPR/Cas9 system is increasingly used for gene inactivation in mouse zygotes, but homology-directed mutagenesis and use of inbred embryos are less established. In particular, Rosa26 knock-in alleles for the insertion of transgenes in a genomic 'safe harbor' site, have not been produced. Here we applied CRISPR/Cas9 for the knock-in of 8-11 kb inserts into Rosa26 of C57BL/6 zygot...

Phosphorylation of mouse p53 at Ser18 occurs after DNA damage. To determine the physiological roles of this phosphorylation event in p53-dependent DNA damage responses, a Ser18 to Ala missense mutation was introduced into the germline of mice. Thymocytes and fibroblasts from the knock-in mice show reduced transactivation of many p53 target genes following DNA damage. p53 protein stabilization a...

The core of many workflow processes in banks, insurance companies, governmental departments, and administrations of multinationals is formed by a set of tasks that are used to classify cases into two groups: accepted and rejected. Each of these Ž . tasks has two possible outcomes: OK or NOK i.e., Not OK . If for a specific case all tasks result in OK, the case is accepted, otherwise it is rejec...

OSCAR is a web platform for cluster and crossspecies analysis of microarray data. It provides a comprehensive but friendly environment to both users and algorithm developers. For users, OSCAR provides cluster tools for both single and multiple species data, together with interactive analysis features. For single species data, OSCAR currently provides Hierarchical Clustering, K-means, partition ...

Previous studies with peritoneal macrophages obtained from macrophage scavenger receptor class A (MSR-A) knock-out mice showed that the endocytic uptake of advanced glycation end products (AGE) by macrophages was mediated mainly by MSR-A. However, it is controversial whether the endocytic uptake of intravenously injected AGE proteins by liver sinusoidal endothelial cells (LECs) is similarly exp...

Engine knock is an undesired phenomenon in spark ignited internal combustion engines; such as a gasoline engine running the Otto cycle. Knocking originates from abnormal combustions at timings other than those decided by controlled ignition. Individual abnormal combustions of this type is also referred to as auto-ignition. Knocking is not only a limitation on engine efficiency and thus of envir...