Calotropis procera, a plant of family Asclepiadaceae is of immense importance as multipurpose plant with medicinal as well as traditional uses. Latex of this plant has been used to cure disease like leprosy, syphilis, inflammation, cutaneous infections, and malarial fever and in many more. In vitro culture of this pharmacologically plant is required otherwise it leads the segregated loss of des...

Lallemantia iberica (Bieb.) Fisch. and C. A. Mey is high valued annual ornamental and medicinal plant from Lamiaceae family that prefers dry sunny hillsides, roadsides, slopes, and fallow fields over an altitude of 500-2150 m. It bears beautiful white flowers and bloom from April to June each year. This study reports L. iberica micropropagation using cotyledon node explants isolated from 15-day...

In vitro budinduction and shoot regeneration of Lantana camara L. was investigated using axillary nodal explants. Multiple shoot buds were induced on Murashige and Skoog (MS) media supplemented with 0, 2, 4 and 8 mg/l of 6-benzylaminopurine (BAP) or thidiazuron (TDZ) for proliferation and 0, 0.25 and 0.5 mg/l indole-3 butyric acid (IBA) for rooting. After 4 weeks, a maximum number of shoot buds...

Penelitian ini bertujuan untuk menganalisis pengaruh pemberian ekstrak tomat dan BAP terhadap respon pertumbuhan in vitro epikotil jeruk siam Pontianak. merupakan penelitian eksperimental menggunakan Rancangan Acak Lengkap (RAL) Faktorial dengan dua faktor. Faktor pertama, konsentrasi (T) taraf perlakuan, T0: kontrol, T1: 5%, T2: 10%, T3: 15%. kedua, (B) B0: 0 mg/l, B1: 0,5 B2: 1 B3: 1,5 mg/l. ...

The present study focuses on the development of a micropropagation protocol for true to type plants Anubias heterophylla, valuable ornamental aquatic plant. Natural propagation species is limited due production small number with long cultivation period, disease and requirement large space propagation. Surface-disinfected shoot tip explants were cultured MS medium containing 0.1 % (w/v) activate...

In vitro propagation of strawberry was conducted at the Biotechnology Lab. of BARI, Joydebpur, Gazipur. For shoot induction, five BAP concentrations viz., 0.0 (Control), 0.5, 1.0, 1.5, and 2.0 mg/l and for root induction four IBA concentrations viz., 0.0 (Control), 0.5, 1.0, and 1.5 mg/l were used. The highest average number of shoots (7) and the highest average length (3.34 cm) of shoot was ob...

The induction and improvement of in vitro rhizogenesis of microshoots of Prosopis chilensis (Mol.) Stuntz and Nothofagus alpina (Poep. et Endl. Oerst.) were compared using Agrobacterium rhizogenes (Ar) versus indole-3-butyric acid (IBA) in the culture media. Microshoots of P. chilensis (1-2 cm length), coming from in vitro grown seedlings, were cultivated in a modified Broadleaved Tree Medium (...

Rice (Oryza sativa L.) is the world’s most important cereal crop. The Doairgura variety (O. a low-yielding, high-quality aromatic rice-producing in Bangladesh. This study was undertaken to develop an vitro regeneration protocol through callus induction. MS media supplemented with different concentrations of 2, 4- D (0.5, 1.0, 2.0 and 3.0 mg /l) were used for induction from mature dehusked rice ...

A method for tissue culture of micropropagation aquatic plant Staurogyne repens (Nees) Kuntze has been developed. Different phytohormones were investigated to optimize the growth explants. The shoot tip explants S. placed in MS nutrient medium including BAP (0.30-1.50 mg/l) + TDZ (0.10 NAA and Kn mg/l). Shoot regeneration frequencies changed as 77.77-100% 66.66-100% cultivation augmented with B...