We describe a cell-free chromatin assembly system derived from the yeast Saccharomyces cerevisiae, which efficiently packages DNA into minichromosomes in a reaction dependent on exogenous core histones and an ATP-regenerating system. Both supercoiled and relaxed plasmid DNA serve as templates for nucleosomal loading in a gradual process that takes at least 6 h for completion at 30 degrees C. Mi...

Several unit-length minicircles from the kinetoplast DNA of Leishmania tarentolae were cloned into pBR322 and into M13 phage vectors. The complete nucleotide sequences of three different partially homologous minicircles were obtained. The molecules contained a region of approx. 80% sequence homology extending for 160-270 bp and a region unique to each minicircle . A 14-mer was found to be conse...

The Munich chain-ladder method for claims reserving was introduced by Quarg and Mack on an axiomatic basis. We analyze these axioms, and we define a modified Munich chain-ladder method which is based on an explicit stochastic model. This stochastic model then allows us to consider claims prediction and prediction uncertainty for the Munich chain-ladder method in a consistent way.

Let G be a subdivision of a ladder graph. In this paper we study magic evaluation with type (1, 1, 1) for a given any general ladder graph G. We prove that subdivided ladder admits magic evaluation having type (1, 1, 1). We also prove such a subdivision admits consecutive magic evaluation having type (1, 1, 0). AMS (MOS) Subject Classification Codes: 05C78

This paper describes the first use of frequency-domain fluorescence lifetime for multiplex detection of DNA restriction fragments in capillary electrophoresis (CE). The fragments were labeled with monomeric intercalating dyes that can be excited by either the 488- or 514-nm line of an argon ion laser and have lifetimes in the range of 0.5-2.5 ns. We were able to achieve multiplex lifetime detec...

The ladder graph plays an important role in many applications as Electronics, Electrical and Wireless communication areas. The aim of this work is to present a new class of odd graceful labeling for the ladder graph. In particular, we show that the ladder graph Ln with m-pendant Ln mk1 is odd graceful. We also show that the subdivision of ladder graph Ln with m-pendant S(Ln)  mk1 is odd grace...

We study the consistency of the ladder approximation and the rainbow approximation of the Dyson-Schwinger equation of QCD. By considering the non-Abelian property of QCD, we show that the QED-type Ward-Takahashi identity is not required for the rainbow-ladder approximation of QCD. It indicates that there does not exists any internal inconsistency in the usual rainbow-ladder approximation of QCD...

BACKGROUND Guidelines for hypertension vary in their preference for initial combination therapy or initial monotherapy, stratified by patient profile; therefore, we compared the efficacy and tolerability of these approaches. METHODS AND RESULTS We performed a 1-year, double-blind, randomized controlled trial in 605 untreated patients aged 18 to 79 years with systolic blood pressure (BP) ≥150 ...

Detecting changes in receptor binding at the metabotropic glutamate receptor 5 (mGluR5) with the PET allosteric antagonist, [¹¹C]ABP688, may be valuable for studying dysfunctional glutamate transmission associated with psychiatric illnesses. This study was designed to validate the findings of a recent pilot study in baboons which reported a significant global decrease from baseline [¹¹C]ABP688 ...