To investigate whether offspring sex ratio at birth (SRB) was associated with maternal breast cancer risk, the present case-control study and a meta-analysis of literature were done. A total of 389 female breast cancer patients who had at least one offspring participated in the study. From general population 356 healthy female were selected as a control group. Control subjects were frequency ma...

There is accumulating evidence that the ability of actinomycetes to produce antibiotics and other bioactive secondary metabolites has been underestimated due to the presence of cryptic gene clusters. The activation of dormant genes is therefore one of the most important areas of experimental research for the discovery of drugs in these organisms. The recent observation that several actinomycete...

Measurements of the back scattering by bluntly-truncated tilted cylindrical shells in water reveal a dependence on the aspect angle which can be interpreted using geometrically described coupling mechanisms [S.F. Morse, P.L. Marston, and G. Kaduchak, J. Acoust. Soc. Am. 103, 785-794 (1998)]. By exciting a shell with a suitable acoustic pulse localized in time, the recorded backscattering respon...

OBJECTIVE The aim of this study is to relate different sociodemographic, health and physical activity parameters to levels of satisfaction with life as a whole and with 10 specific domains of life. DESIGN Data on socio-demographic items were sampled, using strictly structured interviews, while a checklist (LiSat-11) was used for self-reported levels of life satisfaction. SUBJECTS A national...

2.Chen, C. and H. Okayama. 1987. High efficiency transformation of mammalian cells by plasmid DNA. Mol. Cell. Biol. 7:2745-2752. 3.Chu, G.H., H. Hayakawa and P. Berg. 1987. Electroporation for the efficient transfection of mammalian cells with DNA. Nucleic Acids Res. 15:1311-1326. 4.Felgner, P.L., T.R. Gadek, M. Holm, R. Roman, H.W. Chan, M. Wenz, J.P. Northrop, G.M. Ringold and M. Danielsen. 1...

Infections are an important cause for morbidity and mortality in pediatric acute myeloid leukemia (AML). We therefore characterized infectious complications in children treated according to the trial AML-BFM 2004. Patients with Down syndrome were excluded from the analysis. Data were gathered from the medical records in the hospital where the patients were treated. A total of 405 patients (203 ...

Here we describe an error-compensating kinetic-based method for the enzymatic quantification of creatinine in serum. The method, which has a large linear range and very low dependency on experimental variables that influence enzyme activity, is based on the use of creatinine amidohydrolase in a four-step coupled reaction sequence to generate a product that is monitored photometrically. We colle...

Gaoyou duck (Chinese indigenous breed) is famous for double-yolked egg. In the present study, SNP of prolactin (PRL) gene intron 1 of Gaoyou duck was detected by PCR-RFLP and DNA sequencing. The results indicated that there were 2 Dra I recognition sites in intron 1 of the PRL gene, but only 1 was of polymorphism. A T/C mutation at the position of the 1326 bp of the PRL gene was found by DNA se...

A total of 65 maize grain samples , from 7 maize producing areas of Pakistan, were assayed for 14 toxicologically significant mycotoxins viz., aflatoxin B1 (AfB1), aflatoxin B2 (AfB2), aflatoxin G1 (AfG1), aflatoxin G2 (AfG2), zearalenone (ZON), deoxynivalenol (DON), 3acetyl-deoxynivalenol (3A-DON), 15acetyl-deoxynivalenol (15A-DON), nivalenol (NIV), T-2 toxin (T-2), HT-2 toxin (HT-2), diacetox...

Amylases from Streptomyces are useful in the production of maltooligosaccharides, but they have weak thermal stability at temperatures higher than 40 °C. In this study, α-amylase (SAV5981 gene of Streptomyces avermitilis) was expressed from Streptomyces lividans 1326 and purified by ammonium sulfate fractionation followed by anionic chromatography (Q-HP sepharose). The properties of the purifie...