OBJECTIVE To evaluate the effect of chlorhexidine and ethanol on microleakage of composite resin restoration to dentine. METHODS Class II cavities with dentinal margin were prepared on 96 premolar teeth. All specimens were acid-etched, rinsed and dried. Then the samples were randomly divided into four groups according to pre-treatment of the dentine: no treatment (control group); treatment wi...

Aim. To assess the effectiveness of nanosilver gel (NSG) in comparison to chlorhexidine gluconate (CHX) and camphorated phenol (CP) against Enterococcus faecalis (E.f) biofilm. Methods and Materials. Two tests were done, methyl thiazolyl tetrazolium (MTT) assay and confocal laser scanning microscopy (CLSM) analysis, to determine the effectiveness of NSG, CHX, and CP on E.f biofilm. Polystyrene ...

Protein expression and fatty acid profiles of biofilm cells of chlorhexidine-tolerant Delftia acidovorans (MIC = 15 µg/ml) and its chlorhexidine-susceptible mutant (MIC = 1 µg/ml) were investigated. The chlorhexidine-susceptible mutant (MT51) was derived from the parental strain (WT15) using Tn5 transposon mutagenesis. The disrupted gene was identified as tolQ, a component of the tolQRAB gene c...

1990s [7–9] . We and other study groups have shown that a significant proportion (5–10%) of IgE-mediated anaphylaxis occurring during general anaesthesia is triggered by chlorhexidine [8, 10, 11] . To put this into perspective, the incidence rate of peri-operative (all causes) anaphylaxis during anaesthesia has been estimated to be 1 in 10,000–20,000 in France and Australia [12, 13] . Hong et a...

Microbial contamination of plastic surfaces is a significant source hospital-acquired infections. To produce antimicrobial surfaces, chlorhexidine was attached to nitrided acrylonitrile butadiene styrene (ABS). The uniformity distribution on the revealed by time-of-flight secondary ion mass spectrometry (TOF-SIMS) imaging. Its efficacy established against model pathogenic Gram-positive and Gram...

A gloved-hand wash method was used to compare the antimicrobial effect of chlorhexidine gluconate alcohol emollient hand wash (HIBISTAT) with that of 70% isopropyl alcohol on the normal flora of the hands (81 subjects) under conditions designed to mimic use by surgeons. Results of the immediate postwash effects on the bacterial counts for all 3 tests days showed that chlorhexidine significantly...

Introduction: Historically, countless compounds in aqueous solution have been suggested as root canal irrigants. MTAD shows promise as an endodontic irrigant but there are very few studies comparing its antimicrobial effectiveness with NaOCl and Chlorhexidine. Objectives: To compare the antibacterial efficiency of MTAD, 2.5% Sodium Hypochlorite and 2% Chlorhexidine root canal irrigants against ...

BACKGROUND Ventilator-associated pneumonia (VAP) is a nosocomial infection of multifactorial etiology and has a negative influence on cardiovascular surgery (CVS) outcomes. OBJECTIVES Determine the effect of toothbrushing plus 0.12% chlorhexidine gluconate oral rinse in preventing VAP after CVS. METHODS In a quasi-experimental study, patients undergoing heart surgery were enrolled in a prot...

BACKGROUND Chlorhexidine is widely used as an antiseptic agent. It is a potentially allergenic substance that can cause severe hypersensitivity reactions. OBJECTIVE We describe six patients who had anaphylactic reactions attributed to chlorhexidine during surgery. These patients were exposed to chlorhexidine in gels, swabs and catheters. MATERIALS AND METHODS Six patients from three UK cent...

We previously reported that the in vitro antimicrobial activity of a 2.0% chlorhexidine endodontic irrigant was equivalent to that of 5.25% sodium hypochlorite. The purpose of this study was to determine if chlorhexidine irrigants could instill substantive antimicrobial activity in instrumented root canals in vitro. Human teeth were instrumented using 2.0% or 0.12% chlorhexidine as irrigants. A...