The ability of alcohols to regulate inositol lipid-specific phospholipase C (phosphoinositidase C) was examined in turkey erythrocyte ghosts prepared by cell lysis of erythrocytes which were prelabeled with [3H] inositol. Guanosine 5'-[gamma-thiotriphosphate] GTP[S] stimulated the production of both [3H]inositol bisphosphate (18-fold) and [3H]inositol trisphosphate (6-fold) in this system. The ...

Bergsten, Peter. Glucose-induced pulsatile insulin release from single islets at stable and oscillatory cytoplasmic Ca21. Am. J. Physiol. 274 (Endocrinol. Metab. 37): E796– E800, 1998.—The cytoplasmic Ca21 concentration ([Ca]i) and insulin release were measured simultaneously in mouse pancreatic islets cultured overnight. [Ca]i was 105 nM and insulin release 3 pmol ·g21 ·s21 at 3 mM glucose. An...

The cytoplasmic Ca2+ concentration ([Ca2+]i) and insulin release were measured simultaneously in mouse pancreatic islets cultured overnight. [Ca2+]iwas 105 nM and insulin release 3 pmol ⋅ g-1 ⋅ s-1at 3 mM glucose. An increase to 7 mM glucose reduced [Ca2+]itransiently, whereas insulin release doubled and was pulsatile with a frequency of 0.47 min-1. [Ca2+]i oscillations with similar frequency a...

The gas kinetic temperature ( T K ) determines the physical and chemical evolution of interstellar medium (ISM). This work explores use HCN/HNC (J=1–0) line ratio as new probe in molecular ISM. Based on a set IRAM-30m observations at 30 arcsec resolution towards Orion Nebula Cluster, we find two-part linear correlation observed . empirical calibration allows to obtain direct estimates values ac...

The aim of this work was to investigate the effect of different concentrations of NaCl (0 mM, 100 mM, 200 mM and 300 mM) on germination, seedling growth and acid phosphatase activity of Linum usitatissimum seeds. Flax seeds were germinated under control and saline (100, 200 and 300 mM NaCl) conditions and the effect of salt stress on germination and enzyme activity was determined. Final germina...

Despite the well-known functional importance of GroEL-GroES complex formation during the chaperonin cycle, the stoichiometry of the complex has not been clarified. The complex can occur either as an asymmetric 1:1 GroEL-GroES complex or as a symmetric 1:2 GroEL-GroES complex, although it remains uncertain which type is predominant under physiological conditions. To resolve this question, we stu...