The objective of this study was to identify and evaluate for the first time anticoagulant fibrinogenolytic protease from Psidium guajava seed extract. extract in sodium dodecyl sulfate polyacrylamide gel electrophoresis showed protein bands high molecular weight region between 122 kDa 47 two distinct lower at ~16.6 ~14.3 kDa. content found be 1 mg/ml with specific activity 0.8 U. proteolytic su...

The activators of blood clotting present’ in mammalian tissue, i.e. the agents responsible for the conversion of prothrombin to thrombm, have been shown to be lipoproteins of a very high particle weight (l-3). These substances readily form sediments in a strong centrifugal field (31,OOOg), but remain in solution when subjected to weaker centrifugal forces (5000g). The consideration of the manne...

• Recent studies from this laboratory have shown that the clotting time of blood' and the morphology of blood clots' can be altered markedly by flow of blood during the period of clotting. The rheology of clots has been studied by Hartert, de Nicola, and Marchal et al. by means of thrombelastography, while Scott Blair and Copley and Scott Blair employed a gelometer. However, no observations hav...

We have studied factor VII activation by measuring the ratio of factor VII clotting to coupled amidolytic activity (VIIc/VIIam) and cleavage of 125I-factor VII. In purified systems, a low concentration of Xa or a higher concentration of IXa rapidly activated 125I-factor VII, yielding a VIIc/VIIam ratio of 25 and similar gel profiles of heavy and light chain peaks of VIIa. On further incubation,...

Serum transfusion of 15 to 55ml was done in 30 cases, and prothrombin time, thrombo-test, and plasma clot time were measured at various intervals. A marked shortening of clotting time was seen in 13 of 17 cases with normal coagula bility and 11 of 13 cases of abnormal blood clotting. Favorable clinical effects were seen in 5 of 8 cases of leukemia including a case with oozing from the tongue, a...

Much recent research on blood clotting has dealt with prothrombin and factors which influence its activation. In the development of such investigations experimental procedures have become increasingly complex and artificial. They usually involve the use of anticoagulants, often multistage fractionation by precipitation, adsorption or filtration, and, almost invariably, the addition of tissue ex...

• Recent studies from this laboratory have shown that the clotting time of blood' and the morphology of blood clots' can be altered markedly by flow of blood during the period of clotting. The rheology of clots has been studied by Hartert, de Nicola, and Marchal et al. by means of thrombelastography, while Scott Blair and Copley and Scott Blair employed a gelometer. However, no observations hav...

Surface coatings of the albumin-heparin conjugates were developed to improve the blood compatibility of polymeric materials. Glass, PVC, Biomer and cellulose acetate were coated with albumin-heparin conjugate and its adsorption and desorption behavior on glass in particular was studied using 3H and 51Cr radiolabeled conjugates. Precoated materials showed a significant prolongation of the Lee-Wh...