The formation of an active dinitrogenase requires the synthesis and the insertion of the iron-molybdenum cofactor (FeMo-co) into a presynthesized apodinitrogenase. In Azotobacter vinelandii, NafY (also known as protein) has been proposed to be a FeMo-co insertase because of its ability to bind FeMo-co and apodinitrogenase. Here we report the purification and biochemical characterization of NafY...

In order to investigate the effect of different levels of nitrogen and bio-fertilizers on yield of canola plants in Qazvin, Iran, a split-plot design was conducted on the base of randomized complete block design with four replications. Treatments included 3 levels of nitrogen (0, 75 and 150 kg ha-1) and bio-fertilizers on four levels (no inoculation, Azotobacter, Azospirillum and a combination ...

Azotobacter vinelandii cultures express more H2 uptake hydrogenase activity when fixing N2 than when provided with fixed N. Hydrogen, a product of the nitrogenase reaction, is at least partly responsible for this increase. The addition of H2 to NH4+-grown wild-type cultures caused increased whole-cell H2 uptake activity, methylene blue-dependent H2 uptake activity of membranes, and accumulation...

in order to study the qualitative and quantitative yield and some agronomic characteristics of sunflower (helianthus annus l.) in response to seed inoculation with pgpr under various levels of nitrogen fertilizer, a factorial experiment was conducted based on a randomized complete block design with three replications in field experimental university of mohaghegh ardabili during growing season o...

This study aims to determine the effect of C/N ratio a mixture dairy cow dung and kirinyuh leaves on quality extraction or Liquid Organic Fertilizer (POF). The treatment consisted manure with different C/N, namely P1=16, P2=18, P3=20, P4=22. was decomposed for 14 days then extracted incubated days. Observations included decrease in C-organic content (%), total N-total ratio, P K number P-solven...

Experiments were carried out in manometric vessels of sufficient size to accommodate 18 ml. of reaction mixture. These were made up by using the same proportion of reactants as previously described (1) : 1.20 ml. of 0.2 M phosphate buffer (pH 6.89, 0.6 ml. of 0.02 M MgSOd, 0.6 ml. of 0.025 per cent coenzyme A, 0.6 ml. of 0.1 M sodium acetate-1-C14, 1.2 ml. of 0.1 M sodium citrate, and 3.0 ml. o...

this study was conducted in acompletely randomized block design with three replications using three concentrations of 107, 105, and 103cfu/ml of azotobacter isolated from the rhizosphere of karun varieties of barley (az. salentris) and standard az.chrococom along with control (zero concentration of bacteria). the aim of this study was to study the reactions of barley plantin response to inocula...

Inoculation of azotobacter has significant positive effects on the growth characteristics and pigments in mangrove seedlings of Avicennia marina and Ceriops decandra. The bacterial inoculation significantly increased the root dry biomass at the maximum of 75.8% at 30 gl(-1) salinity in Ceriops decandra. But in Avicennia marina, the shoot dry biomass was increased significantly at the maximum of...