The shikimate pathway enzyme chorismate mutase converts chorismate into prephenate, a precursor of Tyr and Phe. The intracellular chorismate mutase (MtCM) of Mycobacterium tuberculosis is poorly active on its own, but becomes >100-fold more efficient upon formation of a complex with the first enzyme of the shikimate pathway, 3-deoxy-d-arabino-heptulosonate-7-phosphate synthase (MtDS). The cryst...

Cyclitol derivatives have been synthesized and screened for growth inhibitory effect upon prokaryotic and eukaryotic organisms. One derivative, (2S,3R,5R)-3-azido-2-benzoyloxy-5-hydroxycyclohexanone, was studied in detail: it has no effect upon bacteria, but it is inhibitory to Neurospora crassa. In Neurospora crassa it increased the amount of myo-inositol-1-phosphate synthase and inhibited the...

Abstract The herbicides glyphosate and pyrithiobac inhibit the enzyme 5‐enolpyruvylshikimate‐3‐phosphate synthase (EPSPS) in aromatic amino acid biosynthetic pathway acetolactate (ALS) branched‐chain pathway, respectively. Here we characterise protease activity profiles of a sensitive (S), glyphosate‐resistant (GR) multiple‐resistant (MR) population Amaranthus palmeri response to pyrithiobac. A...

Glyphosate is an organophosphate herbicide manufactured by Monsanto, which eliminates annual and perennial weeds inhibiting the enzyme 5-enolpyruvylshikimate-3-phosphate synthase (EPSPS) involved in production of aromatic amino acids plants microorganisms. As this used extensively, there a lot research on its effect plants, animals microbes, human health. contaminates different ecosystems spray...

Salmonella typhimurium mutants conditionally deficient in 3-deoxy-d-manno-octulosonate-8-phosphate (KDO8P) synthase activity play a central role in our understanding of lipopolysaccharide function in enteric bacteria. The detailed characterization of KDO8P synthase from such a mutant, however, has not been previously reported. To address this issue KDO8P synthase from S. typhimurium AG701 and f...

Cystathionine beta-synthase [L-serine hydrolyase (adding homocysteine), EC 4.2.1.22] was studied in cultured skin fibroblasts from two control subjects and three patients with pyridoxine-responsive homocystinuria. In crude cell sonicates, cystathionine synthase activity detected in each mutant line was less than 5% of control values. After differential centrifugation, ammonium sulfate fractiona...