نتایج جستجو برای: 5 ml culture mediumrpmi

تعداد نتایج: 1615953  

F. Najafi S. Eskandari T. Hasanloo

Elicitation with middle-viscous chitosan (30 mg/50 mL) significantly stimulated silymarin synthesis in Silybum marianum hairy root cultures. The root cultures established by infection with Agrobacterium rhizogenes AR15834 showed a potential for production of silymarin. Elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 mL) was used in order to imp...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1965
A D Welch

Among various systems in which induction of new enzyme synthesis can be initiated, that developed by Talalay,'-3 in which the metabolism of a variety of steroids by Pseudomonas testosieroni is induced by either testosterone or certain other steroidal compounds, has been particularly rewarding. Recent studies of this system by Wacker et al.4' 5 have suggested that a complex may be formed between...

2014
Esquivel-Velázquez Marcela Hernández Ricardo Larralde Carlos Ostoa-Saloma Pedro

Herein we report that in vitro experiments with different initial parasite densities (1, 5, and 10 cysts per mL of culture medium) show that cysts at densities of 10 and 5 grow faster than those at 1, and that they release into the culture medium factors which increase the budding rates of the slower lower-density ones. Close contact among the incubated cysts also favors budding, thus suggestin...

Journal: :Antimicrobial agents and chemotherapy 1982
S B Redjeb A Slim A Horchani S Zmerilli A Boujnah V Lorian

A total of 20 patients with symptomatic urinary tract infections (greater than 10(5) colony-forming units of Escherichia coli per ml of urine in addition to pyuria) received 10 mg of ampicillin and 2 liters of fluid daily for 3 days. After 2 days, 16 patients had culture-negative urine without pyuria. A total of 18 similar patients received only 2 liters of fluid for 3 days. On day 4, all had g...

Journal: :research journal of pharmacognosy 2014
t. hasanloo s. eskandari f. najafi

elicitation with middle-viscous chitosan (30 mg/50 ml) significantly stimulated silymarin synthesis in silybum marianum hairy root cultures. the root cultures established by infection with agrobacterium rhizogenes ar15834 showed a potential for production of silymarin. elicitation with medium molecular weight of chitosan (0, 5, 10, 20, and 30 mg/50 ml) was used in order to improve silymarin pro...

Journal: :Journal of clinical microbiology 1980
T P Wong R K Shockley K H Johnston

The growth requirements of 135 clinical isolates of Neisseria gonorrhoeae and six American Type Culture Collection reference strains were examined by using a simple chemically defined medium known as Wong-Shockley-Johnston medium, WSJM. The simple liquid medium supported growth of gonococci from an inoculum of 2 X 10(6) colony-forming units ml-1 and yielded 10(10) colony-forming units ml-1 in 1...

Journal: :Journal of Functional Foods 2022

This study has developed an innovative method for the production of secondary metabolites starting from Cydonia oblonga Mill (quince) pulp callus culture. The qualitative and quantitative content phenolic triterpenic acids quince extract were elucidated by GC–MS, GC, HPLC-DAD-ESI-MSn. was rich 5-O-caffeoylquinic acid (5-CQA), 5-p-coumaroylquinic (5-p-CoQA) maslinic corosolic acid. Quince extrac...

Journal: :The Journal of Cell Biology 1974
Marcia Perlman Jules L. Baum Gordon I. Kaye

The corneal endothelium is a single layer of polygonal cells covering the posterior surface of the cornea. The apical free surface of these cells faces the anterior chamber and the basal surface rests on an unusually thick basement lamina, Descemet's membrane. This membrane is collage-nous (1, 6, 11, 12) and is believed to be secreted by the endothelial cells (6, 13, 18). These cells form the p...

2002
Bei Huang Thomas Dreyer Martin Heidt Julie C.M. Yu Monika Philipp Friedrich W. Hehrlein Norbert Katz Nadia Al-Fakhri Justus Liebig

Objective: In arteriosclerosis and bypass graft stenosis, intimal proliferation is controlled by local and systemic growth factors, such as platelet derived growth factor (PDGF) or insulin. Intimal hyperplasia can be produced in organ culture models. Our aim was to compare neointima formation in two organ culture models of internal mammary artery (IMA) and saphenous vein (SV), with special refe...

2017
Amanda J. Driscoll Maria Deloria Knoll Laura L. Hammitt Henry C. Baggett W. Abdullah Brooks Daniel R. Feikin Karen L. Kotloff Orin S. Levine Shabir A. Madhi Katherine L. O’Brien J. Anthony G. Scott Donald M. Thea Stephen R. C. Howie Peter V. Adrian Dilruba Ahmed Andrea N. DeLuca Bernard E. Ebruke Caroline Gitahi Melissa M. Higdon Anek Kaewpan Angela Karani Ruth A. Karron Razib Mazumder Jessica McLellan David P. Moore Lawrence Mwananyanda Daniel E. Park Christine Prosperi Julia Rhodes Md. Saifullah Phil Seidenberg Samba O. Sow Boubou Tamboura Scott L. Zeger David R. Murdoch Katherine L. O’Brien Orin S. Levine Maria Deloria Knoll Daniel R. Feikin Andrea N. DeLuca Amanda J. Driscoll Nicholas Fancourt Wei Fu Laura L. Hammitt Melissa M. Higdon E. Wangeci Kagucia Ruth A. Karron Mengying Li Daniel E. Park Christine Prosperi Zhenke Wu Scott L. Zeger Nora L. Watson Jane Crawley David R. Murdoch W. Abdullah Brooks Hubert P. Endtz Khalequ Zaman Doli Goswami Lokman Hossain Yasmin Jahan Hasan Ashraf Stephen R. C. Howie Bernard E. Ebruke Martin Antonio Jessica McLellan Eunice Machuka Arifin Shamsul Syed M. A. Zaman Grant Mackenzie J. Anthony G. Scott Juliet O. Awori Susan C. Morpeth Alice Kamau Sidi Kazungu Micah Silaba Ominde Karen L. Kotloff Milagritos D. Tapia Samba O. Sow Mamadou Sylla Boubou Tamboura Uma Onwuchekwa Nana Kourouma Aliou Toure Shabir A. Madhi David P. Moore Peter V. Adrian Vicky L. Baillie Locadiah Kuwanda Azwifarwi Mudau Michelle J. Groome Nasreen Mahomed Henry C. Baggett Somsak Thamthitiwat Susan A. Maloney Charatdao Bunthi Julia Rhodes Pongpun Sawatwong Pasakorn Akarasewi Donald M. Thea Lawrence Mwananyanda James Chipeta Phil Seidenberg James Mwansa Somwe wa Somwe Geoffrey Kwenda Trevor P. Anderson Joanne Mitchell

Background. Antibiotic exposure and specimen volume are known to affect pathogen detection by culture. Here we assess their effects on bacterial pathogen detection by both culture and polymerase chain reaction (PCR) in children. Methods. PERCH (Pneumonia Etiology Research for Child Health) is a case-control study of pneumonia in children aged 1-59 months investigating pathogens in blood, naso...

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