Amylase is an enzyme that catalyzes the breakdown of starch into sugars and plays a pivotal role in a variety of areas like use as digestives, for the production of ethanol and high fructose corn syrup, detergents, desiring of textiles, modified starches, hydrolysis of oil-field drilling fluids, and paper recycling. In the present work, solid state fermentation (SSF) for α -amylase production h...

BACKGROUND The routine measurement of pleural fluid amylase is frequently recommended, but the cost-effectiveness of this procedure is unknown. METHODS To assess the utility of routine measurement of pleural fluid amylase in evaluating pleural effusions, we measured amylase, glucose, lactate dehydrogenase, and protein levels and blood cell counts in 379 patients undergoing thoracentesis durin...

Macroamylasemia is characterized by an elevated serum amylase activity. It results from the circulation of a macromolecular complex consisting of amylase molecule and a serum component, often an immunoglobulin. The present study presents marked elevation of serum amylase level in a 43-year-old male, who had been admitted to our hospital for slight abdomen ache for 4 months. After treatment foll...

Diabetes mellitus is one of the most common endocrine diseases and its type II is the major form of diabetes, accounting for 90% of cases worldwide. The inhibition of carbohydrate hydrolyzing enzymes such as ?-amylase can be an important strategy in the control of blood glucose levels in patients with type II diabetes. In this investigation, three Morus species, M. alba, M. alba var. nigra and ...

New ELISAs for detecting macroamylase or free autoantibodies to amylase were tested with 48 samples that had been characterized by gel chromatography and electrophoresis. The macroamylase ELISA, with anti-IgG or anti-IgA for detection, detected macroamylase in 28 of 33 samples known to contain macroamylase (85% sensitivity), whereas the ELISA for free autoantibody to amylase was positive for on...

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It is now believed that the inhibition of carbohydrate hydrolyzing enzymes (CHEs) in the digestive tract can significantly prolong the overall carbohydrate digestion time and decrease the postprandial hyperglycemia after a meal. Therefore, inhibitors of CHEs can be useful therapeutic approaches in the management of diabetes mellitus, especially in the type 2, and complications associated with t...

Macroamylasemia results from the complexing of serum a-amylase (EC 3.2.1.1;a-1,4-glucan 4-glucanohydrolase) to a high-molecular-weight protein or proteins (macroamylase) and is characterized by hyperamylasemia and low urinary amylase clearance, associated with normal renal function. Recently a simplified detection method has been described for macroamylasemia, in which a small column of Sephade...

Diabetes mellitus is a metabolic disorder characterized by chronic hyperglycemia. The management of the blood glucose level is a critical strategy in the control of diabetes complications. Inhibitors of carbohydrate hydrolyzing enzymes have been useful as oral hypoglycemic drugs for the control of hyperglycemia especially in patients with type II disables mellitus. The goal of the present study...