Two antibiotic resistance peptides, the E-peptide (MRLFV) and the K-peptide (MRFFV) conferring macrolide and ketolide resistance, respectively, were studied in the complex state with bacterial Staphylococcus aureus ribosomes. Interactions of antibiotic resistance peptides with ribosomes were investigated using two-dimensional transferred nuclear Overhauser effect spectroscopy (TRNOESY), suggest...

Studies were initiated to localize the C-reactive protein (CRP) binding site on the coUagen-like region (CLR) of Clq. CRP bound preferentially to the A chain of reduced Clq, in contrast to aggregated immunoglobulin G (Agg-IgG), which reacted preferentially with the C chain. A group of Clq A chain peptides, including peptides identical to residues 81-97, 76-92, and 14-26, respectively, were synt...

Abstract Severe COVID-19, caused by Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2), is characterized widespread inflammation and cytokine storm. A number of viral proteins are capable inducing inflammatory responses, one which open reading frame 3a (ORF3a). ORF3a from SARS-CoV shares 73% homology with SARS-CoV-2 was shown to stimulate NF-κB signaling inflammasome activation in a manner d...

Studies were initiated to localize the C-reactive protein (CRP) binding site on the collagen-like region (CLR) of C1q. CRP bound preferentially to the A chain of reduced C1q, in contrast to aggregated immunoglobulin G (Agg-IgG), which reacted preferentially with the C chain. A group of C1q A chain peptides, including peptides identical to residues 81-97, 76-92, and 14-26, respectively, were syn...

Abstract Background Accurate prediction of binding between class I human leukocyte antigen (HLA) and neoepitope is critical for target identification within personalized T-cell based immunotherapy. Many recent tools developed upon the deep learning algorithms mass spectrometry data have indeed showed improvement on average predicting power HLA-peptide interaction. However, their performances sh...

The protein CsaA has been proposed to function as a protein secretion chaperone in bacteria that lack the Sec-dependent protein-targeting chaperone SecB. CsaA is a homodimer with two putative substrate-binding pockets, one in each monomer. To test the hypothesis that these cavities are indeed substrate-binding sites able to interact with other polypeptide chains, we selected a peptide that boun...

neurokinin 1 receptors (nk1r) are overexpressed on several types of important human cancer cells. substance p (sp) is the most specific endogenous ligand known for nk1rs. accordingly, a new sp analogue was synthesized and evaluated for detection of nk1r positive tumors. [6-hydrazinopyridine-3-carboxylic acid (hynic)-tyr8-met(o)11-sp] was synthesized and radiolabeled with 99mtc using ethylenedia...

Historical perspective A series of experiments done in the mid 1950s established the heart as an endocrine organ. First, Kisch and colleagues detected secretory granules in guineapig atria. Henry and Pearce subsequently described increased urinary flow after balloon stretch of the canine left atrium. In an experiment done 25 years later, de Bold injected homogenised atrial tissue into rats and ...