Objective(s): Three-dimensional biomimetic scaffolds have widespread applications in biomedical tissue engineering due to similarity of their nanofibrous architecture to native extracellular matrix. Co-culture system has stimulatory effect on chondrogenesis of adult mesenchymal stem cells. This work presents a co-culture strategy using human articular chondrons and adipose-derived stem cells (A...

Major traumatic injuries to the body, such as large surface area burns, limit the availability of autologous stem cell populations for wound repair. This report demonstrates that even after severe burn trauma to the body, resident stem cells present within the subcutaneous adipose tissue survive and are available for therapeutic uses. Debrided skin from wounded areas contains subcutaneous adipo...

Background: Adipose-derived stem cells (ADSCs) are a population of pluripotent cells used for tissue engineering purposes. The main purpose of the present study was to transdifferentiate the ADSCs to Schwann-like cells and to determine the intensity of apoptosis in ADSCs during the transdifferentiation process. Methods: ADSCs were isolated from the inguinal adipose tissue of adult rats and the ...

BACKGROUND Subcutaneous adipose tissue is a rich source of adipose tissue macrophages and adipose-derived stem cells which both play a key role in wound repair. While macrophages can be divided into the classically-activated M1 and the alternatively-activated M2 phenotype, ASCs are characterized by the expression of specific stem cell markers. METHODS In the present study, we have investigate...

background: the aim of this study was optimization of the polyfect gene delivery method of pcdna3.1 expression vector transfected with the mouse pdx-1 gene in three different kinds of mesenchymal stem cells and hepa cells as well as comparison of transfection efficiency leading to expression of the mentioned gene in the cell types used. materials and methods: rat bone marrow-derived mesenchymal...

traffic accident has increased in the last decade. one of the most important outcomes of accident is spinal cord injury (sci). so, cell therapy is obtained a greater attention in most fields of medicine because of its potential for incurable diseases through replacing of dead cells. the current study aims to describe the important of locomotor functional and structural connection of the spinal ...

BACKGROUND The aim of the present study was to investigate whether adipose-derived stem cells could contribute to skeletal muscle-healing. METHODS Adipose-derived stem cells of male rats were cultured and injected into the soleus muscles of female rats. Two and four weeks after injections, muscles were tested for tetanic force (50 Hz). Histological analysis was performed to evaluate muscle co...

BACKGROUND Adipose-derived stem cells are recognized as being an effective mesenchymal stem cell population with enormous potential in different fields of regenerative medicine and stem cell therapy. Although there is unanimous agreement on the harvesting procedure for adipose tissue, there are various protocols for adipose-derived stem cell isolation. The aim of this study was compare two meth...