Guardeño L.M., Quiles A., Llorca E., Pertusa J., Hernando I. (2013): Effect of microwave thawing on microstructure and physicochemical stability of low fat white sauces made with soy protein. Czech J. Food Sci., 31: 568–574. The microstructural and physicochemical stability of white sauces made with soy protein and modified waxy maize starch was evaluated after subjecting them to a freezing-tha...

OBJECTIVE We investigated the effects of freeze-thawing on the properties of articular cartilage. DESIGN The reproducibility of repeated biomechanical assay of the same osteochondral sample was first verified with 11 patellar plugs from 3 animals. Then, 4 osteochondral samples from 15 bovine patellae were divided into 4 groups. The reference samples were immersed in phosphate-buffered saline ...

1. The rigor which takes place when completely frozen frog sartorius muscle is thawed ("thaw rigor"), is accompanied by a decrease in length of 70 per cent and a loss in weight of 35 per cent, whether the muscle is frozen in the resting or the exhausted condition, or during isometric tetanus. Muscle tetanized to maximal shortening shows a loss in weight of 25 per cent on thawing. 2. A load of 8...

OBJECTIVE To evaluate the effect of magnetic-activated cell sorting in cryopreservation-thawing protocols on sperm motility and cryosurvival rate. DESIGN Prospective-controlled study. SETTING Andrology department at a university-based medical institution. PATIENT(S) Ten healthy volunteer sperm donors. INTERVENTION(S) Sperm populations were separated using annexin-V magnetic-activated ce...

Freeze-storage of epididymal sperm is an important technique for the preservation of gametes in animals, including those becoming extinct. We froze canine sperm recovered from the cauda epididymis and investigated the fertility. The qualities of sperm from the cauda epididymis before freezing were: mean sperm motility, 89.4 +/- 1.6 (SE) %; sperm viability, 89.1 +/- 1.1%; and these were signific...

INTRODUCTION The aim of this study was to investigate the stability of a mixture of temocillin 20mg/ml in 5% dextrose and in 0.9% sodium chloride polyolefin bags after freezing, microwave thawing and long-term storage at 5±3°C. METHODS The stability of ten polyolefin bags containing 20mg/ml of temocillin, five bags in 5% dextrose and five bags in 0.9% sodium chloride, prepared under aseptic c...

OBJECTIVE To evaluate the developmental competence of vitrified human oocytes thawed using two different methods to establish an effective cryopreservation protocol. DESIGN In vitro model study. SETTING University-affiliated hospital. PATIENT(S) Patients who underwent a long protocol of ovarian stimulation with GnRH and gonadotropins. INTERVENTION(S) Vitrified oocytes from the patients ...

Background To create hypothesis, which can give a logical explanation related to the benefits of freezing/thawing embryos. Cryopreservation is not only a technology used for storing embryos, but also a method of embryo treatment that can potentially improve the success rate in infertile couples. Methods From the analysis of multiple results in assisted reproductive technology, which have no s...

Characteristics of buffalo semen, diluents used for liquid storage, aspects involved in freezing and thawing of semen are reviewed, and fertility results after artificial insemination (AI) with frozen-thawed semen are given.

Aim . To evaluate the viability of mononuclear cells (MNCs) in leukocyte concentrates (LCs) at stages their preparation, freezing, and thawing. Materials methods The study material included 44 LCs from donors allogeneic hematopoietic stem (HSCs) 189 autologous patients with oncohematological disorders. were obtained by leukocytapheresis after mobilization HSCs. frozen dimethyl sulfoxide (DMSO) ...