Agrobacterium rhizogenes transformation is a more rapid method of obtaining transgenic and edited rubber dandelion ( Taraxacum kok-saghyz ) plants than tumefaciens . The hairy root rol genes are present alongside transgenes after transformation, they change the morphology significantly. Although these useful visual markers indicating successful dandelion, modify phenotype induced by target ulti...

It has been suggested that the rol genes of Agrobacterium rhizogenes could play an essential role in the activation of secondary metabolite production in plant transformed cultures. This study investigated whether the content of anthraquinone phytoalexins was changed in callus cultures of Rubia cordifolia transgenic for the 35S-rolB and 35S-rolC genes in comparison with a non-transformed callus...

Tropane alkaloids present a great economic value in particular for pharmaceutical industry. The culture of Datura sp hairy roots obtained by inoculation with A4 Agrobacterium rhizogenes offers promising prospects for alkaloids in-vitro production. The objective of our study is to try optimizing hyoscyamine production exploiting the composition of mineral fraction of the culture media. On the wh...

A hairy roots culture of Stephania suberosa was established using Agrobacterium rhizogenes ATCC15834. The production of dicentrine was found to be (8.92 +/- 0.07) mg/g dry wt on day 35 of culture. Effects of sucrose content, tyrosine, and medium strength on growth and dicentrine production of S. suberosa were investigated. 6% (w/v) sucrose was an optimum content for the growth and dicentrine ac...

To improve the production of chlorogenic acid (CGA) in hairy roots of Platycodon grandiflorum, we induced over-expression of Arabidopsis thaliana transcription factor production of anthocyanin pigment (AtPAP1) using an Agrobacterium rhizogenes-mediated transformation system. Twelve hairy root lines showing over-expression of AtPAP1 were generated. In order to investigate the regulation of AtPAP...

Maesa lanceolata is a medicinal plant, growing in the tropics of Africa and Asia. It produces a mixture of triterpenoid saponins, some of which have medicinal properties that are of commercial interest. For instance, saponins isolated from Maesa lanceolata exert antiviral, haemolytic and antiangiogenic activities [1]. As conventional plant growth and production is not very well controlled, in v...

The extent of phenotypic variation of St. John's wort (Hypericum perforatum L.) plants transformed with wild agropine ATCC 15834 Agrobacterium rhizogenes plasmid was evaluated with respect to the number of rol genes integrations. The transfer of T(L)-DNA to plant explants during each transformation event was incomplete with different rolA, rolB, and rolC copy numbers. Along with typical feature...

Growth and diterpenoid accumulation (salvipisone, ferruginol, aethiopinone and 1-oxoaethiopinone) during the growth cycle of a Salvia sclarea hairy root culture are described. The roots transformed by Agrobacterium rhizogenes (LBA 9402) were cultured in half-strength B5 liquid medium supplemented with 30 g L(-1) sucrose under light (16 h/8 h light/dark). A culture period of 30 days was optimal ...

Investigations have been made to develop an efficient protocol for identification and quantification of phenolic acids and flavonoids in hairy roots (HR) of Hypericum perforatum L. HR were induced from root segments of in vitro grown seedlings from H. perforatum, after co-cultivation with Agrobacterium rhizogenes A4. Transgenic status of HR was confirmed by PCR analysis using rolB specific prim...