Purified microsomal and postmicrosomal ribosomes have been prepared from dog pancreas by a method involving magnesium precipitation and treatment with sodium deoxycholate. The purified ribosomes were shown to be substantially free from contamination with supematant protein in an experiment employing a radioactive supematant fraction. They contain less than 0.1% of digestive enzymes known to be ...

Label-free detection of biomolecules using an optical whispering-gallery mode sensor in a microelectrofluidic channel is simulated. Negatively charged bovine serum albumin is considered as the model protein analyte. The analyte transport in aqueous solution is controlled by an externally applied electrical field. The finite element method is employed for solving the equations of the charged spe...

The potency of various uncouplers for collapsing the lightinduced pH gradient across thylakoid membranes in intact chloroplasts was investigated by time-resolved optical spectroscopy. The thylakoid transmembrane pH gradient (ApH) was monitored indirectly by measuring the rate of cytochrome (Cyt) f reduction following a light flash of sufficient duration to create a sizable ApH. The results show...

objective: with consideration of lethal effects of aflatoxins specially b1 on human health. estimation of aflatoxin-albumin adduct, as an important marker of aflatoxin exposure, seems essential. the aim of this study is optimization of hplc-fluorescence method for measurement of this important marker in blood serum. materials and methods: in this study, blood serum of three groups of rats as a...

Abstract Objectives To investigate the relationship between ratios of C-reactive protein (CRP)/albumin, neutrophil/lymphocyte (NLR), monocyte/lymphocyte (MLR), mean platelet volume (MPV)/platelet and erythrocyte sedimentation rate (ESR)/albumin in pediatric patients diagnosed with community-acquired pneumonia based on severity disease. Methods This retrospective cross-sectional study included 5...

Serum Albumin is the most abundant protein in blood plasma. Its two major roles are maintaining osmoticpressure and depositing and transporting compounds. In this paper Albumin-Ethanol/Water solutionssimulations are carried out by three techniques including Monte Carlo (MC), Molecular Dynamic (MD) andLangevin Dynamic (LD) simulations in aqueous solutions. Investigation the energy changes with t...

Albumin is an expensive drug which imposes relatively high cost on the health care system. Doing ABC analysis in Shahid Motahari Hospital, it was revealed that albumin is categorized in class A. Therefore, the present study aimed to evaluate the pattern of albumin use and the physicians' adherence to evidenced-based albumin guidelines in this large general non-teaching hospital in Shiraz, Iran....

background: hypoalbuminemia may be caused by liver disease, nephrotic syndrome, burns, protein-losing entropathy, malnutrition, and metabolic stress. alterations in albumin in metabolic stress such as cardiac surgery have been previously investigated. we studied serum albumin concentration in children with congenital heart disease and also the association of hypoalbuminemia with mortality and m...

Human Serum albumin as well as other biological products prepared by blood fractionation for clinical purposes, was found to contain different amount of aluminum in different commercially available human albumin solutions. It has been reported that interaction of chemicals with the container material can occur during plasma collection, manufacture and storage, so aluminum is introduced into alb...

abstractin the last step of desolvation method for preparation of albumin nanoparticles, glutaraldehyde (ga) is added to stabilize the newly formed nanoparticles. due to undesirable effects of ga, the objective of this study was to evaluate alternative methods of crosslinking including ultraviolet (uv) irradiation, adding of glucose and combination of both methods. the nanoparticles were prepar...