The mobility of metazachlor [2-chloro-N-(pyrazol-1-ylmethyl)acet-2',6'-xylidide] in sand soil and loamy sand soil was studied in a soil column under laboratory conditions. Commercial metazachlor formulation (Metazachlor 500 suspension concentrate (SC)) and metazachlor immobilized in alginate matrix were used for leaching experiment. The initial concentration of metazachlor in soil for both form...

Human red blood cells were immobilized in an alginate network which was cross-linked with Ca2+ ions. The immobilized cells were stored for longer than 5 weeks at 4 degrees C in an isotonic buffered NaCl solution containing glucose, inosine, adenine, and guanosine for energy supply. The immobilized cells were released from the alginate network by dissolving the matrix with citrate. Both the immo...

It was aimed to develop the matrix type polysaccharide-based transdermal films of nifedipine (NFD) to provide its long term plasma concentration. The mechanical tests were carried out on gel formulations which were utilised in the fabrication of transdermal films to determine the type of polymer (pectin, sodium alginate) and plasticizer (propylene glycol, glycerine) as well as their concentrati...

The pathogenesis of Pseudomonas aeruginosa is at least partially attributable to its ability to synthesize and secrete the siderophore pyoverdin and the two zinc metalloproteases elastase and LasA, and its ability to form biofilms in which bacterial cells are embedded in an alginate matrix. In the present study, a lysophospholipid, 1-palmitoyl-2-hydroxy-sn-glycero-3-phosphate [also called monop...

Alginate beads represent a simple and highly reproducible in vitro model system for diffusion-limited bacterial growth. In this study, alginate beads were inoculated with Pseudomonas aeruginosa and followed for up to 72 h. Confocal microscopy revealed that P. aeruginosa formed dense clusters similar in size to in vivo aggregates observed ex vivo in cystic fibrosis lungs and chronic wounds. Bact...

The effect of Ultra-Violet (UV) irradiation and acridine orange dye on L-glutamic acid producing Bacillus subtilis B. megaterium was investigated. selected mutant strains wild types were immobilized used for production. exposed to UV treated with respectively. survived found reduce as the exposure time concentration mutagens increased in this study. Four mutants two Mutant strain (MAIR4) yielde...

• Introduction • What are alginate dressings? • How do alginate dressings work? • What are the main indications for alginate dressings? • Are there any side effects of alginate dressings? • Is there any difference between brands? • Is alginate an effective haemostat? • Does zinc make a difference in haemostatic performance? • What is the role of alginate dressings in split skin graft donor site...

An enzymatic in vitro alginate polymerization assay was developed by using 14C-labeled GDP-mannuronic acid as a substrate and subcellular fractions of alginate overproducing Pseudomonas aeruginosa FRD1 as a polymerase source. The highest specific alginate polymerase activity was detected in the envelope fraction, suggesting that cytoplasmic and outer membrane proteins constitute the functional ...

Alginate oligosaccharides with different bioactivities can be prepared through the specific degradation of alginate by alginate lyases. Therefore, alginate lyases that can be used to degrade alginate under mild conditions have recently attracted public attention. Although various types of alginate lyases have been discovered and characterized, few can be used in industrial production. In this s...