The development of effective treatment options for munitions contaminants is essential for Department of Defense live fire range management and sustainability. The energetic compounds 2,4,6-trinitotoluene (TNT) and hexahydro-13,5-trinitro-1,3,5-triazine (RDX) have been identified in range soil. Alkaline hydrolysis, initiated through treatment with lime, has been shown to effectively destroy the...

A combination of steam explosion (SE) and alkaline peroxide (AP) used to pretreat bamboo was investigated. Steam explosion at 224 °C for 4 min was applied to bamboo, and the pretreated bamboo was delignified by alkaline peroxide. Enzymatic hydrolysis was compared in the pretreated samples. Steam pretreatment led to remarkable hemicellulose solubilization (63.2%). Lignin solubilization (93.1%) w...

Sulfatases contain an active site formylglycine residue that is generated by post-translational modification. Crystal structures of two lysosomal sulfatases revealed significant similarity to the catalytic site of alkaline phosphatase containing a serine at the position of formylglycine. To elucidate the catalytic mechanism of sulfate ester hydrolysis, the formylglycine of arylsulfatases A and ...

Humic isoJates from different layers of an organic-matter-rich algal sapropel from Mangrove Lake, Bennuda, were subjected to solid-state 15N nuclear magnetic resonance (NMR) spectroscopy in crder to reveal chemical structural interrelationships tbat allow delineation of diagenetic pathways. Amide-N, most likely from peptide-like material, \Vas found to represent the main organic nitrogen fonu i...

Phosphodiesterase activity is a novel property of the still-enigmatic alkaline phosphatase from osseous plate. Bis-(p-nitrophenyl) phosphate was hydrolysed at both pH 7.5 and 9.4 with an apparent dissociation constant (K0.5) of 1.9 mM and 3.9 mM respectively. The hydrolysis of p-nitrophenyl-5'-thymidine phosphate followed hyberbolic kinetics with a K0.5 of 500 microM. For p-nitrophenyl phenylph...

Phenolic compounds in oil wastewater are highly toxic and refractory. Hydrolysis at pH 12 for h makes these more vulnerable to attack destruction. Under the binding precipitation of polyaluminum chloride (PAC) 8, chemical oxygen demand (COD) was significantly reduced by 38%. The simulation found that hydrolysis + flocculation a complex multistep process. COD removal rate mainly controlled prehy...

The Silver SPR chip was modified by alkaline-silane condensation with aminopropyltriethoxysilane (APTES) in NaOH aqueous solution at different times. Silver sputtered slides coated with APTES were immersed in NaOH solution, enabling us to produce silver surfaces homogeneously covered with APTES. The surface properties of grafted APTES on sputtered silver surface as a occasion of time were studi...

In this work, the modifications promoted by alkaline hydrolysis and glutaraldehyde (GA) crosslinking on type I collagen found in porcine skin have been studied. Collagen matrices were obtained from the alkaline hydrolysis of porcine skin, with subsequent GA crosslinking in different concentrations and reaction times. The elastin content determination showed that independent of the treatment, el...

Optimal enzyme mixtures of six Trichoderma reesei enzymes and five thermostable enzyme components were developed for the hydrolysis of hydrothermally pretreated wheat straw, alkaline oxidised sugar cane bagasse and steam-exploded bagasse by statistically designed experiments. Preliminary studies to narrow down the optimization parameters showed that a cellobiohydrolase/endoglucanase (CBH/EG) ra...

BACKGROUND The effectiveness of the enzymatic hydrolysis of cellulose in plant cell wall is strongly influenced by the access of enzymes to cellulose, which is at least in part limited by the presence of lignin. Although physicochemical treatments preceding the enzymatic catalysis significantly overcome this recalcitrance, the residual lignin can still play a role in the process. Lignin is sugg...