An improved method is presented for the purification of 8 alpha-(N1-histidyl)riboflavin, 8 alpha-(N3-histidyl)riboflavin and their 2',5'-anhydro forms, which permits the isolation of sizeable quantities of each of these compounds from a synthetic mixture in pure form. Flavin peptides were isolated from the D-gluconate dehydrogenases of Pseudomonas aeruginosa and Pseudomonas fluorescens and from...

We have developed a reliable high-throughput plasmid isolation system using a 96-well plate format. This system combines a novel glass bead micro-mixing method with modified alkaline lysis and Sephacryl S-500 DNA purification procedures. Mechanical forces generated by vortexing glass beads inside each well of the 96-well plates ensure that the bacterial pellets are homogeneously resuspended, th...

An automated trihydroxyindoleprocedure for the differential estimation of epinephrine and norepinephrinein tissues,urine, and blood is presented in detail. After an initial purification of the catecholaminesby adsorption on aluminum oxide, the neutralized eluates are subjected to automatic analysis, in which potassiumferricyanide and an alkaline stabilizing agent are automatically mixed sequent...

The development of vectors such as P1, P1-derived artificial chromosome (PAC) or bacterial artificial chromosome (BAC) (2,6,8) has permitted the cloning of large DNA fragments. These vectors have become common in largescale sequencing and mapping projects. Therefore, a reliable primer walking protocol for P1 plasmids is desirable. It has been demonstrated previously that sequencing of P1 plasmi...

ABSTRACT Biodiesel production has intensified in recent years and the traditionally applied method for its is homogeneous alkaline transesterification, with formation of esters glycerol, which need to be separated. Also, crude biodiesel needs purified at end reaction remove remaining catalyst, soap, oil, alcohol, can impair engine performance. This process generates large quantities wastewater ...

Cyclodextrin glucanotransferase (CGTase, EC 2.4.1.9) is an unique enzyme capable of converting starch and related substrates into cyclodextrins (CDs). In this paper, we report an one step gel purification method of CGTase from Bacillus sp. and later enzyme characterization. The Bacillus sp. strain was isolated from a Colocacia esculenta rizospheric soil sample and the CGTase production was carr...

The practical feasibility and generic applicability of the direct integration of cell disruption by bead milling with the capture of intracellular products by fluidised bed adsorption has been demonstrated. Pilot-scale purification of the enzyme L-asparaginase from unclarified Erwinia chrysanthemi disruptates exploiting this novel approach yielded an interim product which rivalled or bettered t...

Lactulose is a synthetic disaccharide which has remarkable applications in food and pharmaceutical fields. Lactulose is not found in nature and it is produced by isomerization reaction of lactose in an alkaline environment. It should be noted that this reaction has a very low yield since significant amount of lactose stays unreacted in the system. Basically, purification of lactulose is difficu...

Purified preparations of cattle rhodopsin have been titrated to various pH, irradiated, and the pH changes followed thereafter until completed. In this way we have obtained the titration curves of rhodopsin, of the immediate product of irradiation, measured within 30 seconds; and of the final product of irradiation (opsin). The rhodopsin preparations display about 54 titratable groups per mole ...