Factor VIII (FVIII) is a large glycoprotein that is challenging to express both in vitro and in vivo. Several studies suggest that high levels of FVIII expression can lead to cellular stress. After gene transfer, transgene expression is restricted to a subset of cells and the increased FVIII load per cell may impact activation of the unfolded protein response. We sought to determine whether inc...

The article suggests a model for defining rational range and volume of supply perishable goods in their chains functioning at the time random demand. In verbal formulation, goal is to determine delivery volumes that maximize profits with restrictions on funds available purchase, storage weight, as well lost profits. formalized representation determined by properties supplied goods. If these are...

      Topical and transdermal drug delivery systems are noninvasive and can be self-administered with the minimization of side-effects, have received increased attention during the past few years. Nanoemulsions, emulsions sized between 20-200 nm with narrow distributions, offer several advantages for topical and transdermal delivery of pharmaceutical agents including controlled droplet size, th...

Objective(s): PEI based nanoparticle (NP) due to dual capabilities of proton sponge and DNA binding is known as powerful tool for nucleic acid delivery to cells. However, serious cytotoxicity and complicated conditions, which govern NPs properties and its interactions with cells practically, hindered achievement to high transfection efficiency. Here, we have tried to optimize the properties of ...

In a previous report (M.S. Wadhwa et al. (1997) Bioconjugate Chem. 8, 81-88), we synthesized a panel of polylysine-containing peptides and determined that a minimal repeating lysine chain of 18 residues followed by a tryptophan and an alkylated cysteine residue (AlkCWK18) resulted in the formation of optimal size (78 nm diameter) plasmid DNA condensates that mediated efficient in vitro gene tra...

objective: the objective of this study is to develop and assess targeted pamam-peg nanocarrier with anti-tag72 nanobody for t-bid gene coding construct delivery into the human colonic adenocarcinoma cells. materials and methods: nanobody (nb) coding sequence was subcloned into psj expression vector for large-scale production and then nb was purified by ni++ affinity chromatography. sds-page an...

Gene delivery is a powerful technique that often exploited in the treatment of several diseases.

As a part of our continuing studies on 'Polyamines and their role in human disease' we are investigating how polyamines, and especially how novel polyamine conjugates, interact with DNA. We are studying how these conjugates interact with circular plasmids in order to produce nanometre-sized particles suitable for transfecting cells. Our considerations of structure--activity relationships (SAR) ...

Cationic liposome-DNA (CL-DNA) complexes are being pursued as nonviral gene delivery systems for use in applications that include clinic trials. However, to compete with viral vectors for systemic delivery in vivo, their efficiencies and pharmacokinetics need to be improved. The addition of poly (ethylene glycol)-lipids (PEGylation) prolongs circulation lifetimes of liposomes, but inhibits cell...

The human cytomegalovirus (HCMV) early glycoprotein products of the US11 and US2 open reading frames cause increased turnover of major histocompatibility complex (MHC) class I heavy chains. Since US2 is homologous to another HCMV gene (US3), we hypothesized that the US3 gene product also may affect MHC class I expression. In cells constitutively expressing the HCMV US3 gene, MHC class I heavy c...