In the present study, we aimed to develop and validate a rapid and sensitive, Alu-based real-time PCR method for the detection of circulating cell-free DNA (cfDNA). This method targeted repetitive elements of the Alu reduplicative elements in the human genome, followed by signal amplification using fluorescence quantification. Standard Alu-puc57 vectors were constructed and 5 pairs of specific ...

The in vivo transcription of human Alu family members has been investigated by a sensitive primer extension method. The selected primers represent various regions of the Alu family consensus sequence, thus assaying the transcriptional activity of the entire family rather than the activity of an individual member sequence. Using this method, a very small number of RNA molecules per HeLa cell is ...

CA125 amounts have a large overlap in ovarian cancer and benign diseases. We conducted a retrospective cohort trial to assess the clinical value of circulating cell-free DNA concentration and integrity index for the diagnosis of ovarian cancer. A total of 150 patients were recruited. Plasma samples of 24 ovarian cancer patients, 12 benign ovarian cysts, and 12 healthy controls were assessed. By...

Stream processors are high-performance programmable processors optimized to run media applications. Recent work has shown these processors to be more areaand energy-efficient than conventional programmable architectures. This paper explores the scalability of stream architectures to future VLSI technologies where over a thousand floating-point units on a single chip will be feasible. Two techni...

Reversible circuit designing is the area where researchers are focussing more and more for the generation of low loss digital system designs. Researchers are using the concept of Reversible Logic in many areas such as Nanotechnology, low loss computing, optical computing, low power CMOS design etc. Here we have proposed a novel design approach for a 2-bit binary Arithmetic Logic Unit (ALU) usin...

An Arithmetic Logic Unit (ALU) is the heart of every central processing unit (CPU) which performs basic operations like addition, subtraction, multiplication, division and bitwise logic operations on binary numbers. This paper deals with implementation of a basic ALU unit using two different types of adder circuits, a ripple carry adder and a sklansky type adder. The ALU is designed using appli...

This paper proposed a system-on-chip (SOC) architecture for speech recognition which is speaker dependent. The feature extraction bases on LPC (linear predictive coefficient)-cepstrum coefficients, and template matching employs Hidden Markov Models (HMM). It does not aim to offer a sophisticated solution but rather a high speed solution. This SOC architecture includes an ASIC of LPC-cepstrum an...

With the scaling of technology, need for high performance and more functionality, power dissipation becomes a major bottle neck for microprocessor systems design, because clock power can be significant in high performance systems. We propose a low power ALU for high performance systems, which make use of clock gating to reduce clock power. This low-power ALU based on the observation that while ...

The human albumin-alpha-fetoprotein genomic domain contains 13 repetitive DNA elements randomly distributed throughout the symmetrical structures of these genes. These repeated sequences are located at different sites within the two genes. The human albumin gene contains five Alu elements within four of its 14 intervening sequences. Two of these repeats are located in intron 2, and the remainin...

Alu insertion polymorphisms (polymorphisms consisting of the presence/absence of an Alu element at a particular chromosomal location) offer several advantages over other nuclear DNA polymorphisms for human evolution studies. First, they are typed by rapid, simple, PCR-based assays; second, they are stable polymorphisms-newly inserted Alu elements rarely undergo deletion; third, the presence of ...