Enzyme-substrate binding constants, K’e, for chymotrypsin and specific amide (N-acetyl-Mryptophanamide and Nacetyl-L-phenylalaninamide) and ester (N-acetyl-L-tryptophan ethyl ester) substrates have been measured by a proflavin-displacement method. Also, the rate constant for the formation of one intermediate in the chymotrypsincatalyzed hydrolysis of the ester has been determined at selected pH...

(15)NH(4) (+) and [(15)N](amide)-glutamine externally supplied to detached nodules from soybean plants (cv. Tamanishiki) were incorporated within nodule tissues by vacuum infiltration and metabolized to various nitrogen compounds during 60 minutes of incubation time. In the case of (15)NH(4) (+) - feeding, the (15)N abundance ratio was highest in the amide nitrogen of glutamine, followed by glu...

microwave-assisted three component cyclocondensation reactions of aldehydes, amides and dienophiles in the presence of acetic anhydride and para-toluenesulfonic acid as a catalyst to afford the highly substituted cyclohexene derivatives, in relatively good yields after several minutes are reported.

in this article six new copoly(amide-imide)s (11a-c) were synthesized through the directpolycondensation reaction of 1,3-bis[4,4'-(trimellitimido) phenoxy] propane (6) with 3,3'-diamino diphenyl sulfone (7), 4,4'-diaminodiphenyl ether (8) and 1,5-naphtalene (9) in thepresence of fumaric acid (10) as a second diacid in a medium consisting of n-methyl-2-pyrrolidone, triphenyl phosp...

Hypodentate diamine cobalt(III) pentammine complexes [Co(NH3)5(NH2(CH2)(n)NH3)](ClO4)4 (8: a: n = 3; b: n = 4; c: n = 6; d: n = 8) have been synthesized via the reaction of [Co(NH3)5(OTf)](OTf)2 (TfOH = CF3SO3H) with the corresponding diamines. The analogous t-boc protected diamine complexes [Co(NH3)5(NH2(CH2)(n)NHt-boc)](ClO4)3 (7a-d) were prepared in 4-26% yield. Low yields for the formation ...

The acylation of megakaryocyte proteins was studied with special emphasis on the myristoylation and palmitoylation of the glycoprotein (GP) Ib complex. Guinea pig megakaryocytes were purified and separated into subpopulations at different phases of maturation. Cells were incubated with [3H]myristate, [3H]palmitate, or [3H]acetate to study endogenous protein acylation. Cycloheximide was used to ...

Certain "degradation" products of GLP-1 were found to possess beneficial effects on metabolic homeostasis. Here, we investigated the function of the COOH-terminal fragment of GLP-1, the nonapeptide GLP-1(28-36)amide, in hepatic glucose metabolism. C57BL/6 mice fed a high-fat diet (HFD) for 13 wk were injected intraperitoneally with GLP-1(28-36)amide for 6 wk. A significant reduction in body wei...