We present a coarse-grained model for evaluation of interactions of globular proteins with nanoparticles (NPs). The protein molecules are represented by one bead per aminoacid and the nanoparticle by a homogeneous sphere that interacts with the aminoacids via a central force that depends on the nanoparticle size. The proposed methodology is used to predict the adsorption energies for six common...

Dynamic mutations involve expansion of the number of repeat units consisting of three or more nucleotides in tandem (i.e. adjacent to one another) present in a gene or in its neighborhood. These repeats may occur in different genes and may code for different aminoacids. According to expansions sizes, it is possible to have unaffected individuals that are carriers of a pre-mutation. Instability ...

Abstract Background and Aims Diabetic kidney disease (DKD) is the leading cause of end-stage renal disease, but few biomarkers early identification DKD are available. The aim study was to asses new urinary plasma in stages DKD, especially normoalbuminuric type 2 diabetes mellitus (DM) patients. Method This cross-sectional pilot performed an integrated metabolomic profiling blood urine 90 patien...

The interaction of 11-mercaptoundecanoic acid capped gold nanoparticles (MUA-GNPs) with europium ions and aminoacids has been studied by UV-Vis spectrophotometry, fluorescence, confocal fluorescence microscopy, resonance light scattering and TEM. Results demonstrated that hyper-Rayleigh scattering emission occurs upon the addition of lysine to the MUA-GNPs-Eu(III) system, thus providing an inhe...

-A lectin of M, ca 45,000 per subunit from Amaranthus leucocarpus seeds, has been isolated and purified by affinity chromatography using a stroma column. It is a glycoprotein (10% w/w carbohydrate) containing six N-acetylD-glucosamines, four D-galactoses, one D-glucose and traces of xylose residues for each three D-mannose residues per molecule. Its amino acid composition reveals a predominance...

We have applied solid-phase synthesis methods for the construction of tris(bipyridyl) peptidic ligands that coordinate Fe(ii) ions with high affinity and fold into stable mononuclear metallopeptides. The main factors influencing the folding pathway and chiral control of the peptidic ligands around the metal ions have been studied both by experimental techniques (CD, UV-vis and NMR) and molecula...

Mucoviscidosis is the most common genetic autosomal recessive disease in Caucasian populations, with an incidence of 1/2200 – 2500 live birth and a carrier frequency of 1 in 25 – 30 persons. The disease is caused by mutations of the CFTR gene (cystic fibrosis transductance conductance regulator), which is located on chromosome 7 in locus q31.2. The CFTR gene is a large gene (250 kpb) with 27 ex...

PPE represent a peculiar family of mycobacterial proteins characterized by a 180 aminoacids conserved N-terminal domain. Several PPE genes are co-transcribed with a gene encoding for a protein belonging to another family of mycobacterial specific proteins named PE. Only one PE-PPE couple has been extensively characterized so far (PE25-PPE41) and it was shown that these two proteins form a heter...

The separation and quantitative measurement of aminoacids by ion-exchange chromatography has been in use for many years ]. However, the value of this method is limited by the time required for the analysis: 6 to 23 h. Other methods, such as gas chromatography, give results more rapidly, but do not allow the separation of all amino-acids [2]. The development of cation-exchange resins, capable of...