Trypanothione reductase (TR) is an essential enzyme of trypanosomatids and therefore a promising target for the development of new drugs against African sleeping sickness and Chagas' disease. Diaryl sulfides with a central anilino moiety, decorated with a flexible N-alkyl side chain bearing a terminal ammonium ion, are a known class of inhibitors. Using computer modelling, we revised the bindin...

Positive-ion thermospray liquid chromatography-mass spectrometry (TSP-LC-MS) is used to detect organic acids via the direct injection of untreated urine from newborns and infants. Two methods are reported for the separation of organic acids. The separation of urinary organic acids is effected in either an acidic, pH 2.5 sulfuric acid, or a non-acidic, 0.05 M ammonium acetate, pH 6.8, mobile pha...

The potentiometric response properties of ammonium-, carbonate-, and proton-selective electrodes prepared by incorporating appropriate neutral carriers within novel asymmetric cellulose acetate membranes are reported. The membranes are formed by first casting a thin layer of cellulose triacetate without carrier, hydrolyzing one side of this film with base, and then on the other side casting a s...

In the title compound, C(6)H(19)N(4) (+)·Cl(-)·CH(2)Cl(2), the non-H atoms of the ammonium ion show non-crystallographic C(3) symmetry. The chloride ion is embedded in a framework of seven crystallographically independent hydrogen bonds (five N-H⋯Cl and two C-H⋯Cl), which form layers parallel to the (100) plane. Two N---H...N bonds also occur.

The ammonium permease Mep2p mediates ammonium uptake and also induces filamentous growth in the human-pathogenic yeast Candida albicans in response to nitrogen limitation. The C-terminal cytoplasmic tail of Mep2p contains a signaling domain that is not required for ammonium transport but is essential for Mep2p-dependent morphogenesis. Progressive C-terminal truncations showed Y433 to be the las...