To elucidate a functional significance of submaxillary gland, a simple in vivo biological assay for uterine growth inhibitory activity of submaxillary gland extract has been developed based on its inhibition of serum gonadotropin induced uterine growth of immature mice. Regression of weight gain after administration of serum gonadotropin was found sigmoidal against protein concentration of subm...

An investigation on purification and characterization of amyloglucosidase enzyme from Endomycopsis fibuligera by fermentation of sago starch has been carried out. This enzyme is inductive and can be produced by fermenting sago starch in a medium containing E. fibuligera. Crude enzyme was obtained by centrifuging the medium cultures containing E. fibuligera at 6,000 rpm for 20 min and then addin...

Objective: L-asparaginase has been isolated from a wide variety of microbial as well as plant and animal sources. In the study the production, partial purification and kinetic characterization of L-asparaginase from Pseudomonas fluorescens were carried. Methods: L-asparaginase was produced by submerged fermentation method. The purification of L-asparaginase was done through ammonium sulphate pr...

Phosphorus is one of the major components required for the metabolic activities and for the growth of any organism. Many soil organisms are known to solubilize inorganic phosphates. Aspergillus niger isolated from the soil showed extensive solubilization of Tri-calcium phosphate.It was observed that the solubilization was due to drop in pH.Acidification was due to production of organic acids by...

SINCE the discovery of the xanthine oxidase of milk by Morgan, Stewart and Hopkins [1922] two main attempts have been made to purify this enzyme further; namely, by Dixon and Thurlow [1924, 1], and later by Sbarsky and Michlin [1925]. Dixon and Thurlow obtained an active and stable oxidase preparation by precipitating the enzyme, together with the caseinogen and fat, by halfsaturation of the mi...

An enzymatic biotransformation process based on extracted pyruvate decarboxylase (PDC) overcomes the problem of by-product benzyl alcohol production. Seven methods of cells disruption which included application of glass beads, freezing/thawing sequence, silver nanoparticles, ultrasonication, freezing/thawing sequence with glass beads, freezing/thawing sequence with silver nanoparticles, and fre...

1. Phosphofructokinase from rat liver has been partially purified by ammonium sulphate precipitation so as to remove enzymes that interfere in one assay for phosphofructokinase. The properties of this enzyme were found to be similar to those of the same enzyme from other tissues (e.g. cardiac muscle, skeletal muscle and brain) that were previously investigated by other workers. 2. Low concentra...