The effect of temperature variation on the multiplication rates of two strains of Amoeba proteus and the corresponding heterotransfer strains obtained by transplantation of nuclei between the 'parent' strains, has been studied. The two 'parent' strains multiply at a similar rate at 17° C but at different rates at 27 C and 11° C. The multiplication rates of the heterotransfers follow the cytopla...

Creating a biocomputer with its hardware incorporating biological materials, is it possible to implement some unique functions that are difficult for conventional digital computers to deal with? A living organism is a hierarchically-structured system in which a number of self-organization processes run simultaneously on its multiple levels with their characteristic spatiotemporal scales. Becaus...

We show that Σ4–Amoeba–absoluteness implies that ∀a ∈ R (ω L[a] 1 < ω V 1 ), and hence Σ3–measurability. This answers a question of Haim Judah (private communication).

The mechanism by which trophozoites of the protozoan parasite, Entamoeba histolytica destroy mammalian cells is not clear. A variety of amoeba-associated properties have been correlated with virulence (reviewed in 1 and 2). In vitro studies (3-6) have shown that contact of the trophozoite with a target cell is required. Contact killing has been described (6) as occurring in the sequence: adhere...

Using antigen-purified anti-chicken gizzard polyclonal serum (cd2), which was previously shown to cross-react with plant caldesmon-like protein [1], Amoeba proteus protein extracts were analysed by Western blots. Two closely migrating bands were detected at an apparent molecular weight of approximately 65/66 kDa. The cellular distribution of amoeba-caldesmon in migrating and pinocytosing cells ...

Some artificial chemistries model the synthesis, evolution and complexity of digital life consisting of sequences of computer operations (opcodes) that are driven by point mutations to compete for memory and CPU time. One of us previously built Amoeba, a computer world inspired by Tierra and designed to study the emergence of self-replicating sequences of opcodes from a prebiotic world initiall...

Legionella pneumophila is a ubiquitous fresh-water bacterium which reproduces within its erstwhile predators, environmental amoeba, by subverting the normal pathway of phagocytosis and degradation. The molecular mechanisms which confer resistance to amoeba are apparently conserved and also allow replication within macrophages. Thus, L. pneumophila can act as an 'accidental' human pathogen and c...

Business service engagements involve processes that extend across two or more autonomous organizations. Because of regulatory and competitive reasons, requirements for cross-organizational business processes often evolve in subtle ways. The changes may concern the business transactions supported by a process, the organizational structure of parties participating in the process, or the contextua...

Recent in vitro studies have revealed that a certain Mycobacterium can survive and multiply within free-living amoebae. It is believed that protozoans function as host cells for the intracellular replication and evasion of Mycobacterium spp. under harmful conditions. In this study, we describe the isolation and characterization of a bacterium naturally observed within an amoeba isolate acquired...