The unicellular eukaryote Tetrahymena pyriformis secretes significant amounts of cyclic AMP into its external medium. Cells transferred from growth medium into any of the following three different non-nutrient media: (a) 5 mM phosphate buffer containing 47 mM NaCl and 1 mM MgSO4, (b) 10 mM Tris, or (c) 1.3 mM Tris containing 1 mM citrate and 1 mM Ca(OH)2, released to the outside almost 60--80% ...

Voltage-clamp techniques were used to study the effect of forskolin, 8-Br-cyclic AMP, and phorbol 12,13-dibutyrate (PDBu) on the 3 voltage-dependent calcium current components of mouse dorsal root ganglion neurons in culture. Forskolin and 8-Br-cyclic AMP selectively reduced the transient high-threshold (N-type) calcium current component, an effect also produced by PDBu, a C kinase activator. P...

Abstract. Atmospheric CH4 mole fractions resumed their increase in 2007 after a plateau during the 1999–2006 period, indicating relative changes sources and sinks. Estimating by exploiting observations within an inverse modeling framework (top-down approaches) is powerful approach. It is, nevertheless, challenging to efficiently differentiate co-located emission categories sinks using alone. As...

The effect of different N-N spectator ligands on the reactivity of platinum(II) complexes was investigated by studying the water lability of [Pt(diaminocyclohexane)(H2O)2]2+ (Pt(dach)), [Pt(ethylenediamine)(H2O)2]2+ (Pt(en)), [Pt(aminomethylpyridine)(H2O)2]2+ (Pt(amp)), and [Pt(N,N'-bipyridine)(H2O)2]2+ (Pt(bpy)). Some of the selected N-N chelates form part of the coordination sphere of Pt(II) ...

Abstract. Although natural CO2-rich groundwaters of eastern Belgium have been known for centuries, the exact origin their gas is still unclear. This paper presents results a sampling campaign in (Spa, Stoumont, Malmedy): 30 samples both carbogaseous and non-carbogaseous were analyzed major elements, CO2 content carbon isotopic composition. Among them, 13 also 3He/4He 4He/20Ne ratios. The combin...

Adenosine 5'-phosphate was synthesized with 3H or 14C label specifically located as [1'-3H]AMP, [1'-14C] AMP, [5'-3H]AMP, and [5'-14C]AMP. The synthesis was accomplished from adenine and glucose or adenine and ribose using enzymes from the pentose pathway and/or from the purine salvage pathways. Structural analysis of the compounds confirmed the locations of the radiolabels. The methods provide...

Long-term regulation of the cyclic nucleotide phosphodiesterase of the C-6 rat glioma cell line has been studied. Both the low K(m) and high K(m) activities can be induced by elevation of intracellular cyclic AMP levels following either dibutyryl cyclic AMP or norepinephrine treatment of the cells. The enzymes are maximally induced by 3-4 hr. The presence of either cycloheximide or actinomycin ...

Abstract. Aqueous reactions may turn precursors into light-absorbing and toxic products, leading to air quality deterioration adverse health effects. In this study, we comprehensively investigated eugenol photooxidation (a representative biomass-burning-emitted, highly substituted phenolic compound) in the bulk aqueous phase with direct photolysis, a hydroxyl radical (OH), an organic triplet ex...

The cell wall is a vital and multi-functional part of bacterial cells. For Staphylococcus aureus, an important human bacterial pathogen, surface proteins and cell wall polymers are essential for adhesion, colonization and during the infection process. One such cell wall polymer, lipoteichoic acid (LTA), is crucial for normal bacterial growth and cell division. Upon depletion of this polymer bac...

Johnson, Emmett J. (University of Mississippi Medical Center, Jackson), and Harry D. Peck, Jr. Coupling of phosphorylation and carbon dioxide fixation in extracts of Thiobacillus thioparus. J. Bacteriol. 89:1041-1050. 1965.-A cell-free system from Thiobacillus thioparus which fixes large quantities of C(14)O(2) in the presence of ribose-5-phosphate, adenosine triphosphate (ATP), and Mg(++) has ...