Purpose: To explore the inhibitory effect and mechanism of action CXCR4 on intervertebral disc degeneration.Methods: The expression was assessed in different degrees degenerate tissues. Nucleus pulposus (NP) cells-induced degeneration were obtained using interleukin 1 beta (IL-1β) stimulation. inhibitor, AMD 3100, employed to resist function CXCR4, order determine whether inhibition acted by ta...

We investigated the association between 3 main proinflammatory cytokines [interleukin (IL)-1β and IL-6] and the risk of acute pancreatitis. Polymerase chain reaction-restriction fragment length polymorphism was used to genotype IL-1β+3954 C/T (rs1143634) and IL-1β-511 C/T (rs16944) and IL-6 -174 G/C (rs1800795) and IL-6 -634 C/G (rs1800796). The genotype distributions of IL-1β+3954 C/T (rs11436...

Interleukin‑1β (IL‑1β) has a significant role in osteoarthritis (OA). The purinergic receptor, P2X4, has previously been implicated in IL‑1β secretion. The NLRP1 inflammasome mediates the production of IL‑1β in inflammatory disorders. However, it is unknown whether P2X4 modulates NLRP1‑mediated IL‑1β release. In the present study, the correlation between the P2X4 receptor and NLRP1 was investig...

Background and Aims: Polycystic ovary syndrome (PCOS) is an endocrine and metabolic disease in females of reproductive age and is a significant infertility cause. Inflammation plays a crucial role in the pathogenesis of PCOS. The present study evaluated whether sitagliptin, dipeptidyl peptidase-4 inhibitor, attenuates inflammatory markers C-reactive protein (CRP), interleukin(IL)-6, tumor necro...

In this study we investigated the implication of NLRP3 inflammasomes in the pathogenesis of idiopathic pulmonary fibrosis (IPF) and rheumatoid arthritis-usual interstitial pneumonia (RA-UIP).NLRP3 inflammasome activation at baseline and following stimulation with lipopolysaccharide/ATP was evaluated by measuring interleukin (IL)-1β and IL-18 levels released in the bronchoalveolar lavage fluid (...

Interleukin-1β (IL-1β) mediates many of the behavioral responses to infection and inflammation, and IL-1β-converting enzyme (ICE) processes intracellular IL-1β, leading to its maturation and secretion. Here we demonstrate that intracerebroventricular injections of lipopolysaccharide (LPS) produced a greater reduction in both food intake and food-motivated behavior in wild-type compared with ICE...

Purpose. To investigate how multipotent retinal stem cells (RSCs) isolated from mice respond to the proinflammatory signaling molecules, IL-1β, IL-18, and IL-17A. Materials and Methods. RSCs were cultured in a specific culture medium and were treated with these cytokines. Cell viability was detected by MTT assay; ultrastructure was evaluated by transmission electron microscopy; expression of IL...

BACKGROUND Proinflammatory cytokine interleukin-1β (IL-1β) released from spinal microglia plays an important role in the maintenance of acute and chronic pain states. However, the cellular basis of this action remains poorly understood. Using whole-cell patch-clamp recordings, we examined the action of IL-1β on AMPA- and NMDA-receptor-mediated currents recorded from substantia gelatinosa (SG) n...

background and aims: adenovector gene transfer induces inflammatory response that finally leads to vector removal from delivered site. the effect of hydrodynamic pre-injection on adenovector mediated liver inflammation remained elusive. materials and methods: different mice groups were pre-treated by hydrodynamic saline, dexamethasone or nothing prior to adeno-luciferase administration. their s...

Duchenne muscular dystrophy (DMD) is a severe X-linked recessive muscle disorder caused by mutations in the dystrophin gene. Nonetheless, secondary processes involving perturbation of muscle regeneration probably exacerbate disease progression, resulting in the fatal loss of muscle in DMD patients. A dysfunction of undifferentiated myogenic cells is the most likely cause for the reduction of re...