This pioneering work reports successful androgenic plant development via embryogenesis from microspore calluses in anther cultures and estimation of bioactive metabolites vitro regenerants parent (control) Cambod tea, Camellia assamica ssp. lasiocalyx (Planch MS) cultivar TV19. Anthers bearing microspores at early-to-late uni-nucleate stage were selected to initiate androgenesis. A pre-treatmen...

This study was designed to systematically analyze and evaluate the effects of in vivo blockade of α₁-adrenergic receptors (α₁-ADRs) on the stress-induced disturbance of steroidogenic machinery in Leydig cells. Parameters followed 1) steroidogenic enzymes/proteins, transcription factors, and cAMP/testosterone production; 2) the main hallmarks of stress (epinephrine, glucocorticoids); and 3) tran...

Stojkov NJ, Janjic MM, Baburski AZ, Mihajlovic AI, Drljaca DM, Sokanovic SJ, Bjelic MM, Kostic TS, Andric SA. Sustained in vivo blockade of 1-adrenergic receptors prevented some of stresstriggered effects on steroidogenic machinery in Leydig cells. Am J Physiol Endocrinol Metab 305: E194–E204, 2013. First published May 14, 2013; doi:10.1152/ajpendo.00100.2013.—This study was designed to systema...

BACKGROUND For the last few years, researchers have been interested in developing a method for chemical sterilization which may be a better alternative to surgical castration. An ideal chemical sterilant would be one that effectively arrests spermatogenesis and androgenesis as well as libido with absence of toxic or other side effects. Calcium chloride in various solutions and concentrations ha...

Background: For the last few years, researchers have been interested in developing a method for chemical sterilization which may be a better alternative to surgical castration. An ideal chemical sterilant would be one that effectively arrests spermatogenesis and androgenesis as well as libido with absence of toxic or other side effects. Calcium chloride in various solutions and concentrations h...

Doubled haploid (DH) technology is a powerful tool in plant breeding to reduce the time and costs needed to produce pure lines, the cornerstone of hybrid seed production. This biotechnological alternative to classic methods allows for a reduction of the typical 7–8 inbreeding generations needed to fix a hybrid genotype to only one in vitro generation. It is therefore much faster and cheaper, be...