We report a new technology for in vivo tracking of hematopoietic cells, using fluorescent lipophilic probes. Because the probe is irreversibly bound in the lipids of the cell membrane. substantial numbers of dye molecules can be incorporated per cell and thus substantial signal to noise can be achieved. Although this technology can be used for all hematopoietic cells, these first findings are r...

The recent widespread application of the microbiological assay of folate in serum, whole blood, and red cells has led to many reports on the folate status of infants (Matoth et al., 1964a, b; Matoth, Pinkas, and Sroka, 1965; Vanier and Tyas, 1966), including some on the folate status of premature infants (Shojania and Gross 1964; Strelling et al., 1966; Vanier and Tyas, 1967). This study was de...

Due to rapid economic development in recent years, China has become a major global source of refractory black carbon (rBC) particles. However, surface rBC measurements have been limited, and the lower troposphere suffers from a complete lack of measurements, especially in heavily rBC-polluted regions such as China’s capital, Beijing (BJ). In this study, we present the first concentration measur...

The empirical success of RBC models is often judged by their ability to explain the behavior of a multitude of real macroeconomic variables using a single exogenous shock process. This paper shows that in a model with the same basic structure as the bare bones RBC model, monetary, cost push or preference shocks are equally successful at explaining the behavior of macroeconomic variables. Thus, ...

a 36-year-old woman with right upper quadrant abdominal pain since three months previously and no other significant medical history was referred for evaluation of an abdominal mass. upon clinical examination, a large palpable mass in the mid -upper abdominal area was noted. abdominal ultrasound and spiral ct-scan showed a large hepatic mass in the left liver lobe. the patient was referred for t...

The reversible aggregation of red blood cells (RBC) into linear and three-dimensional structures continues to be of basic science and clinical interest: RBC aggregation affects low shear blood viscosity and microvascular flow dynamics, and can be markedly enhanced in several clinical states. Until fairly recently, most research efforts were focused on relations between suspending medium composi...

Human erythrocytes bearing electroinserted full-length CD4 (RBC-CD4) can bind and fuse with a laboratory strain of human immunodeficiency virus type 1 (HIV-1) or with T cells infected by HIV-1. Here we show that RBC-CD4 neutralize primary HIV-1 strains in an assay of cocultivation of peripheral blood mononuclear cells (PBMC) from HIV-1-infected persons with uninfected PBMC. RBC-CD4 inhibited vi...

Maintenance of a reducing redox balance is a critical physiologic function of red cells (RBC) that can be perturbed in variety of RBC pathologies. Here we describe a new approach to evaluate in vivo RBC redox status using a redox sensitive GFP (roGFP2) sensor under control of a β-globin mini-promoter, directing expression specifically to erythroid cells. RoGFP2 expressing RBCs demonstrate ratio...

For studying plasma and red blood cell (RBc) electrolytes, we have developed a method that allows frequent accurate and reproducible sampling of both plasma and RBC. We determined that for our purposes, a 1.0-nil volume of sample was optimum. Although the 1.0-nil “Class A” Ostwald-Folin blow-out pipet and the standard “Class A” transfer pipet were adequate for sampling plasma, they were not ade...

Nitric oxide (NO) is a signaling molecule of major importance modulating not only the function of the vascular wall but also that of blood cells, such as platelets and leukocytes. The synthesis of NO in the circulation has been attributed mainly to the vascular endothelium. Red blood cells (RBC) have been demonstrated to carry a non-functional NOS and--due to their huge haemoglobin content--hav...