An indirect solid-phase radioimmunoassay (RIA) was applied to titration of serum and cerebrospinal fluid (CSF) antibodies against a variety of viruses including rubella, mumps, measles, herpes simplex, varicella-zoster, and vaccinia. The test used fixed, virus-infected cells as a source of antigen, and conditions for optimal production of viral antigen were determined for each virus-host cell s...

J. Esterhuysen1 andJ. Barrat2 The reference method to determine the antibody level of a serum sample is seroneutralisation. The principle of this technique is to incubate a constant known amount of rabies virus with a constant volume of the serum to be tested. The virus that has not been neutralised by the antibodies is quantified using an inoculation test either in vivo or in vitro. This metho...

An indirect antibody ELISA was developed for rapid and sensitive quantification of skeletal muscle calpastatin. Polyclonal antibodies were raised in rabbits against recombinant calpastatin, corresponding to domains 2, 3, and 4 of bovine skeletal muscle calpastatin. Western blot analysis revealed that these antibodies specifically recognize an immunoreactive calpastatin protein of approximately ...