Responses to injections of various materials into rabbit ear chambers were studied by in vivo microscopy. The acute inflammatory responses provoked by injections of antibody-antigen complexes were both quantitatively and qualitatively different from the responses obtained after injections of either homologous sera or the antigens alone. The sticking of leukocytes to endothelium during these res...

The guinea pig is much like humans in the cells and mediators involved in immediate hypersensitivity reactions. However, the major anaphylactic antibody in this species is IgG1, not IgE. Recently, we have been successful in producing IgE antibody in guinea pigs. The current study examined whether guinea pig IgE antibody could mediate pulmonary smooth muscle contraction. IgE antibody to picryl a...

development of a stool antigen immunoassay to detect helicobacter pylori infection requires monoclonal antibody against the specific antigen. alkylhydroperoxide reductase (ahpc) of helicobacter pylori has been described as a specific and unique enzyme for h. pylori and therefore, both h. pylori ahpc and anti-ahpc could be useful in the development of serologic and stool antigen tests, for detec...

Epitope-G1 of bovine ephemeral fever virus (BEFV) G glycoprotein has been genetically and antigenically conserved among various isolates of BEFV and only reacts with anti-BEFV neutralising antibodies. Therefore, it is a candidate antigen for development of the enzyme linked immunosorbent assay (ELISA) for serological identification bovine ephemeral fever (BEF)-infected animals. The aim of this ...

An established human neurogenic sarcoma cell line pre sented a tumor-associated antigen common to various sarcoma tumors. The antigen was detected by immunofluorescent techniques with the use of the sera of patients bearing such tumors and is located mainly on the cellular surface. The tumor-specific antibody of most sera showed only membrane immunofluorescence while five sera revealed both int...

A kinetic nephelometric method and instrument have been developed for the rapid determination of specific serum proteins by means of immunoprecipitin reactions. The maximum rate of change of scattered light intensity in an antigen-antibody reaction can be made to occur within 60 s after initiation of the reaction and provides a measure of the antigen concentration under antibody excess conditio...

An established human neurogenic sarcoma cell line pre sented a tumor-associated antigen common to various sarcoma tumors. The antigen was detected by immunofluorescent techniques with the use of the sera of patients bearing such tumors and is located mainly on the cellular surface. The tumor-specific antibody of most sera showed only membrane immunofluorescence while five sera revealed both int...