Apical membrane H+ extrusion in the renal outer medullary collecting duct, inner stripe, is mediated by a Na(+)-independent H+ pump. To examine the regulation of this transporter, cell pH and cell Ca2+ were measured microfluorometrically in in vitro perfused tubules using 2',7'-bis(carboxyethyl)-5(6)-carboxyfluorescein and fura-2, respectively. Apical membrane H+ pump activity, assayed as cell ...

Background: Endodontic success depends on the complete cleaning and shaping of root canal. In order to achieve this goal, debris removal is essential. Even with improvements in instrument design, apical extrusion continues be a cause periradicular inflammation. The current study aimed compare amount apically extruded throughout canal instrumentation TruNatomy, ProTap...

1215 The Rockefeller University Press $30.00 J. Cell Biol. Vol. 216 No. 5 1215–1218 https://doi.org/10.1083/jcb.201702116 In most animals, the earliest stages of development are characterized by the organization of cells into a polarized epithelial sheet. Several tissues arise from the early embryonic epithelium through a regulated process called the epithelial–mesenchymal transition (EMT), in ...

The contribution of organic anion transporter OAT2 (SLC22A7) to the renal tubular secretion of creatinine and its exact localization in the kidney are reportedly controversial. In the present investigation, the transport of creatinine was assessed in human embryonic kidney (HEK) cells that stably expressed human OAT2 (OAT2-HEK) and isolated human renal proximal tubule cells (HRPTCs). The tubula...

Introduction: Sodium hypochlorite is a solution used in endodontic treatment, and if an accidental apical extrusion occurs, serious complications may affect soft tissue. Objective: The aim of this article is to present a case of apical extrusion of sodium hypochlorite (NaOCl) during root canal system instrumentation. Case report: A 28-year-old woman sought a local dental service for root canal ...

Calcium absorption in intestine and kidney involves transport through the apical membrane, cytoplasm, and basolateral membrane of the epithelial cells. Apical membrane calcium influx channels have recently been described in rabbit (epithelial calcium channel, ECaC) and rat (calcium transport protein, CaT1). We amplified from human duodenum a 446-base partial cDNA probe (ECAC2) having a predicte...

Mairbäurl, Heimo, Katrin Mayer, Kwang-Jin Kim, Zea Borok, Peter Bärtsch, and Edward D. Crandall. Hypoxia decreases active Na transport across primary rat alveolar epithelial cell monolayers. Am J Physiol Lung Cell Mol Physiol 282: L659–L665, 2002. First published December 14, 2001; 10.1152/ajplung.00355.2001.—Hypoxia has been reported to inhibit activity and expression of ion transporters of al...