A vector derived from an original infectious clone of Turnip mosaic potyvirus (TuMV) has been developed. The vector (p35Tunos-vec01) was made through the creation of a new unique insertion site for foreign genes between the NIb and the CP genes of the viral genome. The jellyfish Green Fluorescent Protein (GFP) and E. coli β-glucuronidase (GUS) were expressed in Arabidopsis thaliana plants infec...

Plants activate glutathione (GSH)-dependent detoxification pathways at biochemical and molecular levels under boron (B) toxicity. Sulfate uptake transport are necessary for GSH biosynthesis in plants. Therefore, the transcriptional regulation of some sulfate transporters was determined this study to clarify importance these leaf root tissues Arabidopsis thaliana toxic B conditions. The expressi...

UNLABELLED • PREMISE OF THE STUDY Cold tolerance is a critically important factor determining how plants will be influenced by climate change, including changes in snowcover and extreme weather events. Although a great deal is known about cold tolerance in Arabidopsis thaliana, it is not highly cold tolerant. This study examined cold tolerance and its genetic diversity in an herbaceous subarc...

In this study we describe a method for the detection of biomolecules (in polypeptide m/z range) directly from surface plant leaves by using Mass Spectrometry Imaging. The plant-pathogen interaction between Arabidopsis thaliana and bacterium Xanthomonas campestris pv. was analyzed comparing infected non-infected leaf discs submitted to mass spectrometry. total area ion distribution calculated bo...

The genes encoding Δ6 desaturase, Δ6 fatty acid elongase, and Δ5 desaturase from the alga, Phaeodactylum tricornutum, were co-expressed in Arabidopsis thaliana under the control of the Brassica napus napin promoter to produce arachidonic acid (ARA; 20:4 Δ 8, 11, ) and eicosapentaenoic acid (EPA; 20:5 Δ 8, 11, 14, ). ARA and EPA accumulated up to 0.5% and 0.05% of total fatty acids, respectively...

Dated molecular phylogenies are the basis for understanding species diversity and for linking changes in rates of diversification with historical events such as restructuring in developmental pathways, genome doubling, or dispersal onto a new continent. Valid fossil calibration points are essential to the accurate estimation of divergence dates, but for many groups of flowering plants fossil ev...

Plant root systems often have complex branching patterns. Models indicate that a complex architecture is only required for the acquisition of immobile resources, such as phosphate; mobile ions, notably nitrate, can be effectively taken up by very restricted root systems. We have tested this prediction using the axr4 mutation of Arabidopsis thaliana, the principal phenotypic effect of which is t...

The centromeres of Arabidopsis thaliana chromosomes contain megabases of complex DNA consisting of numerous types of repetitive DNA elements. We developed a chromatin immunoprecipitation (ChIP) technique using an antibody against the centromeric H3 histone, HTR12, in Arabidopsis. ChIP assays showed that the 180-bp centromeric satellite repeat was precipitated with the antibody, suggesting that ...

Prenylation is a type of post-translational modification in which 15- or 20-carbon lipid added to the carboxyl (C) terminus protein. Arabidopsis thaliana contains PROTEIN PRENYLTRANSFERASE ALPHA SUBUNIT-LIKE (PPAL) gene, encodes protein with homology ?-subunits three known prenylation enzymes, PFT, PGGT, and Rab-GGT. We previously identified two mutations PPAL, one ppal-1, T-DNA insertion fourt...