The purpose of this study was to examine the ul trastructural relationships of cholesterol crystals and apatite deposits in human atherosclerotic lesions. Segments of human aortic atherosclerotic lesions were obtained at autopsy , fixed in gl utaraldehyde and dehydrated without using any organic solvents. The aortic segments were coated with carbon and subjected to various scan ning electron mi...

Linda J. Hazell,* Leslie Arnold, ‡ Dimity Flowers, ‡ Georg Waeg, § Ernst Malle, ¶ and Roland Stocker* *Biochemistry Group, The Heart Research Institute, Camperdown, New South Wales 2050, Australia; ‡ The Department of Pathology, University of Sydney, Sydney, New South Wales 2006, Australia; and the Departments of § Biochemistry and ¶ Medical Biochemistry, Karl-Franzens University, Graz, A-8010,...

Atherosclerosis, once believed to be a result of a slow, irreversible process resulting from lipid accumulation in arterial walls, is now recognized as a dynamic process with reversibility. Liver-directed gene therapy for dyslipidemia aims to treat patients who are not responsive to currently available primary and secondary prevention. Moreover, gene therapy strategies have also proved valuable...

• Atherosclerosis, the lipid-containing intimal lesion with marked fibrosis and calcification, morphologically similar to the human lesion, has been produced consistently in numerous animal species, mostly by feeding diets high in cholesterol and fat, with and without hormonal or other dietary manipulations. However, ulceration and thrombosis, the so-called "complicated" lesions so frequent in ...

Oxidation of low-density lipoproteins (LDL) is a key process in atherogenesis, and vitamin E (alpha-tocopherol, TOH) has received attention for its potential to attenuate the disease. Despite this, the type and extent of TOH oxidation and its relationship to lipid oxidation in the vessel wall where lesions develop remain unknown. Therefore, we measured oxidized lipids, TOH, and its oxidation pr...

Application of photothermal Optical Coherence Tomography (OCT) to detect macrophages in ex vivo rabbit arteries which have engulfed nanoclusters of gold coated iron oxide (nanorose) is reported. Nanorose engulfed by macrophages associated with atherosclerotic lesions in rabbit arteries absorb incident laser (800nm) energy and cause optical pathlength (OP) variation which is measured using photo...

Apolipoprotein (apo) E-deficient transgenic mice develop marked hyperlipidemia and progressive atherosclerotic lesions. To explore whether oxidative modification of lipoproteins is involved in atherogenesis in this murine model, we performed extensive immunocytochemical studies. Atherosclerotic lesions ranging from early fatty streaks to very advanced plaques were examined from the aortic valve...

Coronary artery disease (CAD), including its acute manifestations such as myocardial infarction (MI), is one of the leading causes of morbidity and mortality worldwide, especially in countries where the development of atherosclerotic plaques is closely linked to certain dietary habits (ie, Western diet). For decades, research has focused on deciphering the detailed mode(s) of disease developmen...

It is well known that cancer and hypercoagulability go hand in hand. Most thromboembolism is venous in nature although arterial thrombosis can occur. Arterial thrombosis secondary to malignancy is usually seen in the lower extremities; however, it can also be seen elsewhere. This is a case of bronchogenic carcinoma with no history of typical atherosclerotic risk factors including smoking, diabe...