We propose protein PTB1 : 4W as a good candidate for engineering into a downhill folder. PTB1 : 4W has a probe-dependent thermal unfolding curve and sub-millisecond T-jump relaxation kinetics on more than one time scale. Its refolding rate in denaturant is a non-linear function of denaturant concentration (curved chevron plot). Yet at high denaturant concentration its unfolding is probe-indepen...

The indirect immunoperoxidase method was applied to the rapid diagnosis of mucocutaneous herpes simplex virus infection. Specimens were obtained directly from lesions at various stages in their evolution. The immunoperoxidase method identified herpes simplex virus-infected cells in 91% of vesicular lesions which were positive by standard virological culture. At later stages, the number of cases...

The simplex method is frequently the most efficient method of solving linear programming (LP) problems. This paper reviews previous attempts to parallelise the simplex method in relation to efficient serial simplex techniques and the nature of practical LP problems. For the major challenge of solving general large sparse LP problems, there has been no parallelisation of the simplex method that ...

Vertical impact force during downhill running should be analyzed in terms of risk of injuries caused in hill training of distance runners. In spite that the impact force in running was influenced by some kinematic parameters at the foot contact (Gerritsen et al. 1995), few studies analyzed interactions between ground reaction forces (GRFs) and kinematics during downhill running in detail. The p...

The authors address the problem of downhill protein folding in the framework of a simple statistical mechanical model, which allows an exact solution for the equilibrium and a semianalytical treatment of the kinetics. Focusing on protein 1BBL, a candidate for downhill folding behavior, and comparing it to the WW domain of protein PIN1, a two-state folder of comparable size, the authors show tha...

Cancer gene therapy based on tissue-restricted expression of cytotoxic gene should achieve superior therapeutic index over an unrestricted method. This study compared the therapeutic effects of a highly augmented, prostate-specific gene expression method to a strong constitutive promoter-driven approach. Molecular imaging was coupled to gene therapy to ascertain real-time therapeutic activity. ...

Estimates of knee joint loadings were calculated for 12 normal subjects from kinematic and kinetic measures obtained during both level and downhill walking. The maximum tibiofemoral compressive force reached an average load of 3.9 times body-weight (BW) for level walking and 8 times BW for downhill walking, in each instance during the early stance phase. Muscle forces contributed 80% of the max...

Registration of preoperative CT data to intra-operative video images is necessary not only to compare the outcome of the vocal fold after surgery with the preplanned shape but also to provide the image guidance for fusion of all imaging modalities. We propose a 2D-3D registration method using gradient-based mutual information. The 3D CT scan is aligned to 2D endoscopic images by finding the cor...

Based on the syllogism of formal logic and triangulation theory of algebraic topology, a new method of knowledge representation with 2-simplex called simplex representation is given in this paper. To show differences, the method is compared with others. The simplex representation has special characteristics in representation abilities, expression ability, and representation modality. General Te...