Enzymatic and homogeneous catalysis offer complementary means to produce enantiopure products. Incorporation of achiral, biotinylated aminodiphosphine-rhodium complexes in (strept)avidin affords enantioselective hydrogenation catalysts. A combined chemogenetic procedure allows the optimization of the activity and the selectivity of such artificial metalloenzymes: the reduction of acetamidoacryl...

Under appropriate conditions, oleic acid replaces biotin for several lactic acid bacteria.‘, z This growth-promoting action of oleic acid is not nullified by excess avidin for Streptococcus jaecalis R2 or for several other organisms (Table I). Egg white did, however, block utilization of oleic acid by Lactobacillus arabinosus (Table I). This result is obtained whether oleic acid is added alone,...

Polyelectrolyte multilayer (PEM) films, established using the layer-by-layer (LbL) technique, are attractive for controlled drug delivery in order to improve tissue regeneration. For instance, the LbL-deposition provides the possibility to incorporate biomolecules via electrostatic interactions for drug delivery, which might be moreover combined with a stable biofunctionalization via covalent b...

The first example of an all-organic oligofluorene truxene based distributed feedback laser for the detection of a specific protein–small molecule interaction is reported. The protein avidin was detected down to 1 μg mL 1 using our biotin-labelled biosensor platform. This interaction was both selective and reversible when biotin was replaced with desthiobiotin. Avidin detection was not perturbed...

Single molecule force spectroscopy is a technique that can be used to probe the interaction force between individual biomolecular species. We focus our attention on the tip and sample coupling chemistry, which is crucial to these experiments. We utilised a novel approach of mixed self-assembled monolayers of alkanethiols in conjunction with a heterobifunctional crosslinker. The effectiveness of...

The study was performed on 45 patients with heart transplantation out of which 30 presented serologic cytomegalovirus infection. For histological study we used: hematoxylin eosin, van Gieson, Masson's trichrome, green methyl pironin stains. Immunohistochemistry was performed using ABC technique (avidin biotin complexes or avidin biotin) with the following monoclonal antibodies: CD8, CD4, CD20, ...

The synthesis of a cyclic peptide-Gd(III)DTPA molecule equipped with biotin is presented, yielding a well-defined multivalent MRI contrast agent after its coupling to avidin.