A novel technique using the incorporation of 5-ethynyl-2'-deoxyuridine (EdU) into replicating DNA is described for the analysis of replicating banding patterns of human metaphase chromosomes. Human lymphocytes were synchronized with excess thymidine and treated with EdU during the late S phase of the cell cycle. The incorporated EdU was then detected in metaphase chromosomes using Alexa Fluor® ...

BACKGROUND Pulmonary arterial hypertension is usually fatal due to right ventricular failure and is frequently associated with co-existing left ventricular dysfunction. Endothelin-1 is a powerful pro-fibrotic mediator and vasoconstrictor that is elevated in pulmonary arterial hypertension. Endothelin receptor blockers are commonly used as pulmonary vasodilators, however their effect on biventri...

Banding in obsidian from Big Glass Mountain (BGM), Medicine Lake volcano, California, and Mayor Island (MI), New Zealand, provides a record with a 1/wavenumber power-spectral density and multifractal characteristics. The samples are compositionally homogeneous, with banding defined by variable microlite content (BGM) or vesicularity (MI). In both samples, banding formation is well explained by ...

Mitochondria were prepared from etiolated maize shoots (Zea mays L. var. McNair 508) by homogenization followed by differential centrifugation and equilibrium banding in discontinuous sucrose or Renografin-sucrose gradients. Mitochondria prepared by sucrose banding showed better physiological integrity than those prepared by renografin-sucrose banding, although both procedures yielded mitochond...

Shear-banding is a curious but ubiquitous phenomenon occurring in soft matter. The phenomenological similarities between the shear-banding transition and phase transitions has pushed some researchers to adopt a ‘thermodynamical’ approach, in opposition to the more classical ‘mechanical’ approach to fluid flows. In this heuristic review, we describe why the apparent dichotomy between those appro...

BACKGROUND To make systemic anti-cancer therapy (SACT) preparation more practicable, dose-banding approaches are currently being introduced in many clinical centres. The present study aimed to determine the potential impact of using recently developed National Health Service in England (NHSE) dose-banding tables in a paediatric setting. METHODS Using pharmacokinetic parameters obtained from 3...

Vorticity banding under steady shear flow is observed in a suspension of semiflexible colloidal rods (fd virus particles) within a part of the paranematic-nematic biphasic region. Banding occurs uniformly throughout the cell gap within a shear-rate interval (.gamma-, .gamma+) , which depends on the fd concentration. For shear rates below the lower-border shear rate .gamma- only shear elongation...

carpal fracture-dislocation is regarded as an unusual orthopedic injury and, thus, orthopedic surgeons are less experienced in dealing with and treating these fractures and dislocations. we report a 20-year-old worker man suffering from an unusual carpal fracture-dislocation. there was trans-scaphoid fracture and lunate dislocation with other carpal proximal bones toward volar of the wrist. two...

Gelation kinetics and rheological properties of poly(vinylidene fluoride-co-hexafluoropropylene) [P(VDF-co-HFP)] solutions in methyl-ethyl-ketone 2-heptanone are investigated. Small-angle X-ray scattering measurements indicate that the systems undergo phase separation by a nucleation process. For concentrations between 6 10 wt% copolymer, strain-hardening appears when gels sheared nonlinear reg...