A practical and easily reproducible method for preparing lecithin of a high degree of purity is urgently needed in connection with the use of lecithin in cardiolipin antigens (1, 2). Methods devised for this purpose have been published (3, 4), but the procedures, based on the classic studies of Levene and his coworkers (5, 6), were somewhat tedious and the lecithins so prepared have not always ...

Probiotic formulations containing lecithin phospholipids have been shown to extend their viability. However, the protective effect of and properties mixtures probiotic cells in aqueous media not fully documented. The aim this study was investigate interactions between Bifidobacterium animalis ssp. lactis (Bifido) probiotics lecithin. lecithin-Bifido were dependent on concentration. FTIR Raman s...

introduction : the encapsulation of nutraceuticals in lipid based carriers, such as liposomes, can lead to increasing of bio-active ingredients bioavailability and controlled release, maintaining their stability in different environmental conditions and increasing solubility of hydrophobic active ingredients in aqueous conditions. food grade liposomes are being increasingly used in food industr...

A rapid (30-min) semiautomated continuous-flow procedure is described for use in assessing the phospholipids of the particulate ("lamellar body") fraction of human amniotic fluid. The method is based on measuring the difference in fluorescence of 1,6,-diphenyl-1,3,5-hexatriene added to amniotic fluid before and after micropore filtration. The filtration step removes "lamellar body" particles, w...

The present study was performed to determine whether the degree of lecithin hydrophobicity regulates bile metastability and, therefore, affects the process of cholesterol crystallization. Supersaturated model bile (MB) solutions were prepared with an identical composition on a molar basis (taurocholate/lecithin/cholesterol, 73:19.5:7.5; total lipid concentration 9 g/dl) except for the lecithin ...

Lecithin-cholesterol acyltransferase (LCAT) is a plasma enzyme which catalyzes the transacylation of the sn-2-fatty acid of lecithin to cholesterol, forming lysolecithin and cholesteryl ester. We have recently proposed a covalent catalytic mechanism for LCAT in which lecithin cleavage proceeds via the formation of a transition state tetrahedral adduct between the oxygen atom of the catalytic se...

The incorporation of [2-3H]glycerol and lysolecithin labeled with [2-3H]glycerol into lecithin and its subspecies was investigated in epithelial and muscular layers of rat small intestine. The labeled compounds were administered in two wways: the intraduodenal injection and the injection into femoral vein. The incorporation of glycerol into lecithin was distinctly higher in epithelial layer tha...

the purpose of this study was to evaluate the probable effects of leptin addition in different levels to the semen extender on sperm quality (motility and motility parameters, viability, sperm membrane integrity, and dna damage). semen specimens were evaluated immediately after leptin addition, equilibration time and after thawing the frozen semen. five healthy buffalo bulls (5 ejaculates from ...

cryopreservation is as an effective way to prevent species extinction risk. in this study, we have investigated the protective effect of different concentrations cryoprotectant and effect of different dilution ratios on sperm motility percent and its motility duration after thaw. for this work dimethyl sulfoxide, methanol and ethylene glycol was used as cryoprotectant, each at concentration of ...