BACKGROUND DNA methylation microarrays have become an increasingly popular means of studying the role of epigenetics in cancer, although the methods used to analyze these arrays are still being developed and existing methods are not always widely disseminated among microarray users. METHODS We investigated two problems likely to confront DNA methylation microarray users: (i) batch effects and...

Two batches of frankfurters containing about 55% chicken meat with two different oils (including canola and soybean) were manufactured. Batch 1 included canola oil and Batch 2 included soybean oil in the products. Various analyses were performed to detect the lipid changes of both batches during storage. Fatty acid composition analysis using gas chromatography showed little change in the fatty ...

Two methanotrophic bacteria, Methylobacter albus BG8 and Methylosinus trichosporium OB3b, oxidized atmospheric methane during batch growth on methanol. Methane consumption was rapidly and substantially diminished (95% over 9 days) when washed cell suspensions were incubated without methanol in the presence of atmospheric methane (1.7 ppm). Methanotrophic activity was stimulated after methanol (...

We develop and discuss a methodology for batch-level analysis of hyperspectral stimulated Raman scattering (hsSRS) data sets of human meibum in the CH-stretching vibrational range. The analysis consists of two steps. The first step uses a training set (n=19) to determine chemically meaningful reference spectra that jointly constitute a basis set for the sample. This procedure makes use of batch...

Polyhydroxyalkanoates are known to be temporarily stored by microorganisms in activated sludge, especially in anaerobic-aerobic processes. Due to the problems resulted from the disposals of plastic wastes and excess sludge of wastewater treatment plants, the production of polyhydroxyalkanoates by treating activated sludge and determining the effect of process variables were the main issues of t...

The separation of enantiomers is of great importance due to their possible differences in therapeutic properties. Preferential crystallization in various configurations of coupled batch crystallizers is used as an attractive means to separate the conglomerate-forming enantiomers from racemic mixtures. However, the productivity of such batch processes can be limited by the nucleation of the coun...

This paper describes approaches to improve the performance of one of the most common and increasingly important aspects of the Human Genome Project (HGP) — large-volume, batch comparison of DNA sequence data. This basic comparison operation, usually carried out by the well-known BLAST program on one subject sequence against the internationally available databases of nearly five million target s...