Human factor XI (FXI) is a blood coagulation factor participating in the early phase of the intrinsic pathway of blood coagulation. It circulates in blood as a glycoprotein composed of two identical chains held together by a single disulfide bond between the fourth apple domains. FXI has been expressed in baby hamster kidney (BHK) cells, where it was synthesized as a single-chain molecule that ...

Cells of a genetic variant of the hamster fibroblast line BHK 21 which lack inosinic pyrophosphorylase activity {IPP~ cells) and therefore cannot normally incorporate [H]hypoxanthine were grown in mixed culture with cells of BHK 21 sublines which have inosinic pyrophosphorylase activity (IPP cells). If not in contact with IPP cells, 1PP~ cells do not incorporate added [H]hypoxanthine into nucle...

We investigated the biogenesis of type I cytopathic vacuoles (CPVIs) in Semliki Forest virus (SFV)-infected cells by immunofluorescence and electron microscopy. By using the ts1 mutant of SFV at the restrictive temperature to avoid superinfection, we showed that the multiplicity of infection affects the time of appearance but not the number of CPVIs in a cell. Formation of CPVIs did not require...

The process of adsorption and RNA transcription by vesicular stomatitis virus (VSV) in cells has been followed by using highly labeled preparations of VSV virions. The initial transcription process (primary parental transcription) is rapid and takes about 4 min to develop complete transcripts of the input genome. The optimal cell temperature for in vivo transcription is between 36 and 39.5 C, a...

Whereas defective interfering particles of Sindbis virus are readily produced in BHK-21 cells or chicken embryo fibroblasts by the techniques of serial undiluted passage, similar methods failed to generate such particles in Aedes albopictus cell cultures. In addition, Sindbis virus stocks produced in BHK-21 cells or chicken embryo fibroblasts and which contained defective interfering particles,...