The interactions of acid–base status, waste nitrogen excretion and metabolism in the gulf toadfish Opsanus beta, a ureogenic teleost, were examined by exposing toadfish to a variety of water treatments. Our measurements show that, like other marine teleosts, toadfish rapidly regulate acid–base status by manipulating blood [HCO3]. Several treatments affected nitrogen excretion. The initial stage...

To further the accurate direct potentiometry of plasma electrolyte concentrations, we investigated the effects of solution composition on the residual liquid junction potential (RLJP) during measurement of K+. Assuming that the binding constant between K+ and proteins or bicarbonate is no greater than with Na+, we calculate that the amount of bound K+ can be neglected. A significant RLJP exists...

Ballard, Stephen T., Laura Trout, Zsuzsa Bebök, E. J. Sorscher, and Angela Crews. CFTR involvement in chloride, bicarbonate, and liquid secretion by airway submucosal glands. Am. J. Physiol. 277 (Lung Cell. Mol. Physiol. 21): L694–L699, 1999.—Previous studies demonstrated that AChinduced liquid secretion by porcine bronchi is driven by active Cl2 and HCO3 2 secretion. The present study was unde...

The objective of this study was to measure differences between arterial and venous blood gas parameters and to evaluate whether arterial blood gas values can be estimated from venous blood in Asiatic black bears (ABBs). Twelve healthy captive ABBs (8 males and 4 females; 8-16 years; 76.8-220 kg) were included in this study. The bears were immobilized with medetomidine and zolazepam-tiletamine u...

Renal tubular acidosis (RTA) type 2, also called proximal renal tubular acidosis, is characterized by hyperchloremic metabolic acidosis due to impaired reabsorption of bicarbonate (HCO3) in proximal tubules. It can be due to isolated defect or part of generalized defect (Fanconi syndrome). Rickets/Osteomalacia is more common and treatment is usually difficult. RTA type 3 is designated when comb...

Methane dry reforming (MDR) is of great interest for its efficient consumption greenhouse gases and the production valuable syngas. The interfaces metal boron nitride (BN) are expected to enhance catalytic activity inhibit coke formation. Herein, composite layered oxides (NiMgAlOx) BN was constructed form interface-confined NiMgAlOx/BN (Ni-MAO/BN) catalysts, unique promotion effects a triple in...

The physiological effects of guanylin (GN) and uroguanylin (UGN) on fluid and electrolyte transport in the teleost fish intestine have yet to be thoroughly investigated. In the present study, the effects of GN, UGN, and renoguanylin (RGN; a GN and UGN homolog) on short-circuit current (Isc) and the transport of Cl-, Na+, bicarbonate (HCO3-), and fluid in the Gulf toadfish (Opsanus beta) intesti...

A new in vitro assay was developed and critically characterized to measure the rate of CO2 excretion by trout red blood cells (RBCs) from HCO3- in their natural plasma under normal in vivo conditions of acid-base status. The assay is based on the addition of [14C]bicarbonate to the whole blood and collection of the resultant 14CO2 in the overlying gas phase. The assay simulates the exposure of ...

Burnham, Charles E., Michael Flagella, Zhaohui Wang, Hassane Amlal, Gary E. Shull, and Manoocher Soleimani. Cloning, renal distribution, and regulation of the rat Na-HCO3 2 cotransporter. Am. J. Physiol. 274 (Renal Physiol. 43): F1119–F1126, 1998.—We recently reported the cloning and expression of a human kidney Na-HCO3 2 cotransporter (NBC-1) (C. E. Burnham, H. Amlal, Z. Wang, G. E. Shull, and...

Acidic extracellular pH (pHe) is characteristic of the tumor microenvironment. Several reports suggest that increasing pHe improves response immune checkpoint inhibitors in murine models. To increase pHe, either sodium bicarbonate (NaHCO3) or citric acid/potassium-sodium citrate (KNa-cit) was chronically administered to mice. It hypothesized ions (HCO3−), produced from these alkalinizing agents...